MEMS particle sorting actuator and method of manufacture
View Patent ↗A MEMS-based system and a method are described for separating a target particle from the remainder of a fluid stream. The system makes use of a unique, microfabricated movable structure formed on a substrate, which moves in a rotary fashion about one or more fixed points, which are all located on one side of the axis of motion. The movable structure is actuated by a separate force-generating apparatus, which is entirely separate from the movable structure formed on its substrate. This allows the movable structure to be entirely submerged in the sample fluid.
1. A microfabricated particle sorting actuator capable of motion and fabricated on a substrate, comprising:
a plurality of microfabricated fluidic channels formed in a plane of the substrate;
a movable structure with a motion substantially in the plane, acted on by a force substantially in the same plane and wherein the movable structure simultaneously opens a first microfabricated fluidic channel which is in the plane and closes a second microfabricated fluidic channel also in the plane and wherein the motion is substantially rotary, and the movable structure is attached to the substrate at one or more fixed points, and further comprising one or more flexible springs attached to the one or more fixed points, which return the movable structure to a first position when the force is removed; and
a sample fluid containing a mixture of cells and flowing in the microfabricated fluidic channels, wherein the movable structure directs a target cell into the first microfabricated fluidic channel and other cells into the second fluidic channel.
2. The microfabricated particle sorting actuator of claim 1 , wherein the movable structure is substantially surrounded by the same fluid which is within the microfabricated fluidic passages; and wherein this fluid contains the mixture of cells.
3. The microfabricated particle sorting actuator of claim 1 , further comprising a magnetically permeable material inlaid into the movable structure, wherein the magnetically permeable material moves in response to a flux generating apparatus disposed outside the substrate, so as to direct the target cell into the first microfabricated fluidic channel.
4. The microfabricated particle sorting actuator of claim 1 , wherein the movable structure has an axis substantially parallel to the motion of the movable structure, wherein that motion is about one or more fixed points, and wherein the one or more fixed points are all located on one side of the axis, and wherein the movable structure moves from a first position to a second position in response to a force arising within the movable structure itself, without mechanical coupling to a force-generating apparatus, and wherein the movable structure opens the first microfabricated fluidic passage when in the second position to direct the target cell into the channel, when the movable structure moves in response to the force, and wherein the movable structure and microfabricated fluidic passages are all disposed substantially in the plane.
5. The microfabricated particle sorting actuator of claim 1 , wherein the motion is substantially linear, and the movable structure is attached to the substrate at two or more fixed points, and wherein the motion directs the target cell into the first microfabricated fluidic channel in one position, while allowing the particle mixture to pass into the second microfabricated fluidic channel in another position.
6. The microfabricated particle sorting actuator of claim 4 , further comprising at least one magnetically permeable fixed feature located adjacent to the movable structure and shaped to concentrate lines of magnetic flux in the vicinity of the movable structure.
7. The microfabricated particle sorting actuator of claim 6 , wherein the at least one magnetically permeable fixed feature comprises at least one of a single magnetically permeable fixed feature with a tapered shape and two magnetically permeable fixed features disposed substantially parallel to one another and substantially symmetrically about the axis of the movable structure.
8. The microfabricated particle sorting actuator of claim 1 , further comprising at least one sympathetic beam, which expands a fluid region directly downstream of the movable structure, allowing fluid to flow into the expanded fluid region when the movable structure moves in response to the force, in order to direct the target cell into the first microfabricated fluidic channel.
9. The microfabricated particle sorting actuator of claim 1 , wherein the entire movable structure is submerged in a sample fluid containing a mixture of cells including a target cell, and the sample fluid flows past the movable structure without the addition of a sheath fluid, and wherein the movable structure moves to direct the target cell into the first microfabricated fluidic channel when diverted, and into the second microfabricated fluidic channel when quiescent.
10. The microfabricated particle sorting actuator of claim 1 , wherein a ratio of a depth of the movable structure compared to a width is at least 1.2.
11. The microfabricated particle sorting actuator of claim 1 , further comprising a transparent material disposed over the plurality of microfabricated fluidic channels, enclosing the sample fluid with the mixture of cells within the microfabricated fluidic channels.
12. A particle sorting system which sorts a target cell from other components of a fluid stream, comprising:
a detection region located in one of the microfabricated fluidic channels upstream of the microfabricated particle sorting actuator of claim 1 ; and
a detection system which generates a signal in response to the detection of a target particle within the detection region, wherein the microfabricated particle sorting actuator diverts the target cell into a particular one of the plurality of microfabricated fluidic channels when moving from a first position to a second position in response to the signal, and allows other components to flow into another of the microfabricated fluidic channels.
13. The particle sorting system of claim 12 , wherein the detection system uses a least one of a mechanical, optical, electrical, photovoltaic, magnetic, mass, hydrodynamic attributes of the particles to distinguish the target cell from other components of the fluid stream.
14. The particle sorting system of claim 12 , wherein the detection system comprises at least one laser, at least one dichroic mirror, and at least one optical detector, and wherein the target particle is identified by at least one of scattered incident light and a fluorescent tag which emits light when irradiated by the at least one laser and wherein the fluorescent tag is affixed to the target cell.
15. The particle sorting system of claim 14 , wherein the laser and the detector share an optical path which is oriented substantially perpendicularly to the plane.
16. The particle sorting system of claim 12 , further comprising:
a source of magnetic flux positioned adjacent to the movable structure, but not mechanically coupled to the movable structure, which interacts with the movable structure to create a magnetic force between the movable structure and the source of magnetic flux, moving the movable structure from the first position to the second position which diverts the target cell into the particular one of the plurality of microfabricated fluidic channels.
17. The particle sorting system of claim 12 , wherein the target cell is at least one of a cancer cell, a T-cell, a sperm cell and a stem cell.
18. The particle sorting system of claim 12 , further comprising:
a disposable, self-contained cartridge which includes the microfabricated particle sorting actuator, fluidic channels, and the detection region, as well as a sample reservoir, a sort reservoir, and a waste reservoir; wherein the sample reservoir is configured to store a sample of a biological fluid containing at least one target cell.