Polypeptides having cellobiohydrolase I activity and polynucleotides encoding same
The present invention relates to polypeptides having cellobiohydrolase I activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having cellobiohydrolase I activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide in an expression host, and wherein the polypeptide has at least 99% sequence identity with the sequence of amino acids 1 to 452 of SEQ ID NO: 16.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by at most four amino acids from amino acids 1 to 452 of SEQ ID NO: 16.
3. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by four amino acids from amino acids 1 to 452 of SEQ ID NO: 16.
4. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by at most three amino acids from amino acids 1 to 452 of SEQ ID NO: 16.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by three amino acids from amino acids 1 to 452 of SEQ ID NO: 16.
6. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by at most two amino acids from amino acids 1 to 452 of SEQ ID NO: 16.
7. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by two amino acids from amino acids 1 to 452 of SEQ ID NO: 8.
8. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity differs by one amino acid from amino acids 1 to 452 of SEQ ID NO: 16.
9. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity comprises the sequence of amino acids 1 to 452 of SEQ ID NO: 16.
10. The nucleic acid construct of claim 1 , wherein the polypeptide having cellobiohydrolase I activity is a variant comprising a substitution, deletion, and/or insertion of one or more amino acids of amino acids 1 to 452 of SEQ ID NO: 16.
11. A recombinant expression vector comprising the nucleic acid construct of claim 1 .
12. A recombinant host cell comprising the nucleic acid construct of claim 1 .
13. A transgenic plant, plant part or plant cell transformed with the nucleic acid construct of claim 1 .
14. A method of producing a polypeptide having cellobiohydrolase I activity, comprising: (a) cultivating the recombinant host cell of claim 12 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
15. The method of claim 14 , wherein the polypeptide having cellobiohydrolase I activity comprises the sequence of amino acids 1 to 452 of SEQ ID NO: 16.
16. The method of claim 14 , wherein the polypeptide having cellobiohydrolase I activity is a variant comprising a substitution, deletion, and/or insertion of one or more amino acids of amino acids 1 to 452 of SEQ ID NO: 16.
17. A method of producing a polypeptide having cellobiohydrolase I activity, comprising: (a) cultivating the transgenic plant or the plant cell of claim 13 under conditions conducive for production of the polypeptide; and (b) recovering the polypeptide.
18. The method of claim 17 , wherein the polypeptide having cellobiohydrolase I activity comprises the sequence of amino acids 1 to 452 of SEQ ID NO: 16.
19. The method of claim 17 , wherein the polypeptide having cellobiohydrolase I activity is a variant comprising a substitution, deletion, and/or insertion of one or more amino acids of amino acids 1 to 452 of SEQ ID NO: 16.