Flow cytometry for high throughput screening
The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus.
1. A method for analyzing a plurality of samples, comprising:
moving a plurality of samples comprising particles in a flow stream to and through a flow cytometer, said flow stream including a separation fluid between adjacent ones of said plurality of samples separating said samples from each other in said flow stream, said flow stream thereby constituting a fluid-separated sample flow stream; and
operating said flow cytometer to focus and detect said plurality of samples in said fluid-separated sample flow stream and to selectively analyze the particles in each of said plurality of samples as said fluid-separated sample flow stream passes through said flow cytometer,
the operating of said flow cytometer including focusing and detecting said separation fluid as different components from said samples.
2. The method of claim 1 , further comprising generating first data points from detections of said separation fluid and second data points from detections of said samples.
3. The method of claim 2 , further comprising comparing said first data points and said second data points.
4. The method of claim 2 , further comprising generating a graph to enable a comparison of said first data points and said second data points.
5. A method for analyzing a plurality of samples, comprising:
guiding a fluid-separated sample flow stream including separated samples and separation fluid to and through a flow cytometer; and
operating said flow cytometer to focus and detect said separated samples in said fluid-separated sample flow stream and to selectively analyze particles in each of said separated samples as said fluid-separated sample flow stream passes through said flow cytometer,
the operating of said flow cytometer including focusing and detecting said separation fluid as different components from said samples.
6. The method of claim 5 , further comprising generating first data points from detections of said separation fluid and second data points from detections of said separated samples.
7. The method of claim 6 , further comprising comparing said first data points and said second data points.
8. The method of claim 6 , further comprising generating a graph to enable a comparison of said first data points and said second data points.