Desiccated biologics and methods of preparing the same
The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same.
1. A method of preserving a red blood cell or platelet consisting of:
contacting the red blood cell or platelet with at least one membrane penetrable sugar and at least one membrane impenetrable sugar; and
drying the red blood cell or platelet at a temperature from about 0° C. to about 40° C. for about 1 hours to about 4 hours to a final moisture content from 5% to 90%.
2. The method of claim 1 wherein the membrane penetrable sugar is trehalose.
3. The method of claim 1 wherein the membrane impenetrable sugar is dextran.
4. The method of claim 1 wherein the membrane penetrable sugar is trehalose and the membrane impenetrable sugar is dextran.
5. The method of claim 1 wherein the moisture content is from about 15% to about 40%.
6. The method of claim 1 wherein the moisture content is from about 20% to about 25%.
7. The method of claim 1 wherein the drying is by vacuum desiccation.
8. The method of claim 7 wherein the drying occurs at a temperature from about 32° C. to about 34° C. for about 3 hours.
9. The method of claim 1 wherein when a platelet is preserved, the membrane penetrable sugar is trehalose, the membrane impenetrable sugar is dextran, and the moisture content is about 15%.
10. The method of claim 1 wherein when a red blood cell is preserved, the membrane penetrable sugar is trehalose, the membrane impenetrable sugar is dextran, and the moisture content is about 25%.