Isolated nucleotide sequences encoding GDF traps
In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.
1. An isolated nucleic acid comprising a nucleic acid sequence that hybridizes to the complement of nucleotides 76-396 of SEQ ID NO:30 in the presence of 6.0×sodium chloride/sodium citrate (SSC) at 45° C. followed by a wash in 0.2×SSC at 65° C., wherein the nucleic acid sequence encodes a polypeptide that comprises an acidic amino acid at the position corresponding to position 79 of SEQ ID NO:1.
2. The isolated nucleic acid of claim 1 , wherein the nucleic acid hybridizes to the complement of the nucleotide sequence of SEQ ID NO:30 in the presence of 6.0×sodium chloride/sodium citrate (SSC) at 45° C. followed by a wash in 0.2×SSC at 65° C.
3. The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises nucleotides 76-396 of SEQ ID NO:30.
4. The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO:30.
5. A cultured cell comprising the nucleic acid of claim 1 , wherein the nucleic acid sequence is exogenous.
6. The cultured cell of claim 5 , wherein the cell is a mammalian cell.
7. The cultured cell of claim 6 , wherein the cell is a CHO cell.
8. The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises a nucleotide sequence encoding a polypeptide comprising amino acids 26-132 of SEQ ID NO:26.
9. The isolated nucleic acid of claim 1 , wherein the nucleic acid comprises a nucleotide sequence encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:26.
10. The isolated nucleic acid of claim 1 , wherein the nucleic acid further comprises a nucleotide sequence encoding a tissue plasminogen activator (TPA) leader sequence.
11. The isolated nucleic acid of claim 1 , wherein the nucleic acid is a recombinant nucleic acid.
12. The isolated nucleic acid of claim 1 , wherein the nucleic acid is operably linked to one or more regulatory sequences.
13. An expression vector comprising the isolated nucleic acid of claim 1 .
14. A cultured cell comprising the expression vector of claim 13 , wherein the cell is a mammalian cell.
15. The cultured cell of claim 14 , wherein the cell is a CHO cell.