Compositions comprising cells expressing cold- and menthol-sensitive receptor (CMR1) polypeptides
The present invention relates to regulation of cold sensation and pain. More particularly, the present invention is directed to nucleic acids encoding a member of the transient regulatory protein family, CMR1, which is involved in modulation of the perception of cold sensations and pain. The invention further relates to methods for identifying and using agents that modulate cold responses and pain responses stimulated by cold via modulation of CMR1 and CMR1-related signal transduction.
1. A composition comprising an isolated or recombinant cell that expresses a cold- and menthol-sensitive receptor (CMR1) polypeptide having at least 95% sequence identity to SEQ ID NO:1 or SEQ ID NO:3 and at least one compound that activates said receptor and elicits cold or menthol responsiveness.
2. The composition of claim 1 , wherein said CMR1 polypeptide has at least 95% sequence identity to SEQ ID NO:1.
3. The composition of claim 1 , wherein said CMR1 polypeptide has at least 95% sequence identity to SEQ ID NO:3.
4. The composition of claim 1 , wherein said compound is a menthol, eucalyptol, or icilin compound.
5. The composition of claim 1 , wherein said composition includes a means for detecting the activation of said CMR1 polypeptide by said compound.
6. The composition of claim 1 , wherein the composition includes a means for detecting a change in intracellular calcium.
7. The composition of claim 6 , wherein the means comprises a fluorescent compound or voltage-sensitive dye.
8. The composition of claim 6 , wherein the composition includes a means for detecting a change in membrane potential.
9. The composition of claim 1 , wherein the CMR1 polypeptide is encoded by a nucleic acid having at least 95% identity to SEQ ID NO: 2 or 4.
10. The composition of claim 1 , wherein the CMR1 polypeptide is encoded by a nucleic acid having at least 98% identity to SEQ ID NO: 2 or 4.
11. The composition of claim 1 , wherein the CMR1 polypeptide has the sequence of SEQ ID NO:3.
12. The composition of claim 1 , wherein the CMR1 polypeptide has the sequence of SEQ ID NO:1.
13. The composition of claim 1 , wherein the cell is a CHO cell line or a Xenopus oocyte.
14. The composition of claim 1 , wherein the composition is in contact with a patch clamp.
15. The composition of claim 1 , wherein the cell is a mammalian cell or an oocyte.