Microarray based sample detection system
A microarray assembly for detection of a target molecule is disclosed. The microarray assemblies comprise an array chamber having a microarray located therein and features that facilitate liquid movement within the array chamber. Also disclosed are methods for making the microarray assembly using rollable films and methods for detecting microarray spots using an internal control fluorophore in the array spot.
1. A microarray assembly for detection of a target molecule in a sample, comprising:
an array chamber with a sample inlet, a sample outlet, a top interior surface, a bottom interior surface, side walls and a microarray located on the bottom interior surface, wherein the array chamber is designed to allow amplification of nucleic acids in the array chamber and hybridization of amplified nucleic acid to the microarray and wherein the top interior surface is a hydrophilic surface that facilitates complete filling of the array chamber by an aqueous fluid;
a waste chamber comprising a waste inlet and an absorbent material; and
a channel having an expansion section with a first end proximate to the outlet of the array chamber and a second end proximate to the inlet of the waste chamber,
wherein the expansion section of the channel has a reversed funnel shape from the first end to the second end, wherein the cross-sectional area of the channel increase continuously in a stepwise manner from the first end of the expansion section to the second end of the expansion section.
2. The microarray assembly of claim 1 , wherein the channel further comprises a switchback section between the expansion section and the inlet of the waste chamber.
3. The microarray assembly of claim 2 , wherein the switchback section comprises two sharp turns in the channel.
4. The microarray assembly of claim 1 , wherein the microarray is an oligonucleotide microarray.
5. The microarray assembly of claim 1 , wherein the microarray is a gel spot microarray.
6. The microarray assembly of claim 1 , wherein the gel spot array comprises a plurality of gel spots and wherein each gel spot comprises an internal control fluorophore and a capturing agent that binds specifically to the target molecule.