Identification and molecular characterisation of proteins, expressed in the
The invention relates to a new polynucleotide which encodes a polypeptide expressed in the salivary glands of ticks, more particularly the Ixodes ricinus arthropod tick, during the slow-feeding phase of the blood meal have, said polynucleotide and related polypeptide may be used in different constructions and for different applications which are also included in the present invention.
1. A method of inhibiting factor XIIa bound to a polyanionic surface, comprising causing the factor XIIa bound to the polyanionic surface to contact an isolated fusion protein which comprises an isolated polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36.
2. The method of claim 1 , wherein the isolated polypeptide is modified by or linked to at least one substitution group.
3. A method of inhibiting thrombus formation in the blood of a human patient, the method comprising:
(A) a method of inhibiting thrombus formation in the blood of a human patient wherein the blood contacts an exposed structure other than the patient's endothelium, the method comprising introducing to the patient's blood a sufficient amount to inhibit thrombus formation of an isolated fusion protein which comprises a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36; or
(B) a method of inhibiting thrombus formation, wherein the formation is inhibited after cutting the patient's vasculature, comprising cutting the patient's vasculature and introducing to the patient's blood flow a sufficient amount to inhibit thrombus formation of an isolated fusion protein which comprises a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36.
4. The method of claim 3 , wherein the polypeptide is modified by or linked to at least one substitution group.
5. A method of inhibiting the contact phase pathway, comprising:
causing a human's blood to contact a sufficient amount to inhibit the contact phase pathway of an isolated fusion protein which comprises a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36; and
exposing the human's blood to a trigger selective for the contact phase pathway.
6. The method of claim 5 , wherein the blood to which the isolated fusion protein is administered is venous blood.
7. The method of claim 5 , wherein the isolated fusion protein is administered as a pharmaceutical composition comprising a pharmaceutical carrier and the polypeptide.
8. The method of claim 5 , wherein the isolated fusion protein is administered by injection.
9. The method of claim 8 , wherein the causing contact comprises administering to the human's blood a pharmaceutical composition comprising the isolated fusion protein and an anti-oxidant, buffer, bacteriostat, or solute that renders the composition isotonic with the blood.
10. The method of claim 5 , wherein the polypeptide is selected from the group consisting of a polypeptide having up to 3 amino acid substitutions relative to the amino acid sequence of SEQ ID NO:36, a polypeptide having up to 3 amino acid deletions relative to the amino acid sequence of SEQ ID NO:36, and a polypeptide having up to 3 amino acid additions relative to the amino acid sequence of SEQ ID NO:36.
11. The method of claim 5 , wherein the KPI domain comprises the amino acid sequence of amino acid residues 40 to 58 of SEQ ID NO:36.
12. The method of claim 5 , wherein the polypeptide further comprises Cys at a position corresponding to position 12 of SEQ ID NO:36, Cys at a position corresponding to position 21 of SEQ ID NO:36, Cys at a position corresponding to position 37 of SEQ ID NO:36, and Cys at a position corresponding to position 62 of SEQ ID NO:36.
13. The method of claim 5 , wherein the polypeptide has the amino acid sequence of SEQ ID NO:36.
14. The method of claim 5 , wherein the polypeptide is modified by or linked to at least one substitution group.
15. The method of claim 5 , wherein the trigger comprises a polyanionic surface.
16. The method of claim 5 , wherein the exposing comprising cutting the human's vasculature after the isolated fusion protein is administered.
17. The method of claim 5 , wherein the causing contact comprises administering to the human's blood by injection a pharmaceutical composition comprising a pharmaceutical carrier and the isolated fusion protein.
18. The method of claim 15 , wherein the polypeptide is modified by or linked to at least one substitution group.
19. A method of preventing clot formation in the blood of a human patient without interfering with the bleeding time, the method comprising:
(A) a method of preventing clot formation in the blood of a human patient without interfering with the bleeding time, wherein the blood contacts an exposed structure other than the patient's endothelium, the method comprising introducing to the patient's blood a sufficient amount to inhibit thrombus formation of an isolated fusion protein which comprises a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36; or
(B) a method of preventing clot formation in the blood of a human patient without interfering with the bleeding time, wherein the formation is inhibited after cutting the patient's vasculature, comprising cutting the patient's vasculature and introducing to the patient's blood flow a sufficient amount to inhibit thrombus formation of an isolated fusion protein which comprises a polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:36 fused to a heterologous polypeptide, wherein said polypeptide has a kunitz-type-protease-inhibitor (KPI) domain, wherein the KPI domain of the polypeptide comprises Phe at position corresponding to position 40 of SEQ ID NO:36, Gly at position corresponding to position 44 of SEQ ID NO:36; Cys at position corresponding to position 45 of SEQ ID NO:36, Phe at position corresponding to position 52 of SEQ ID NO:36, and Cys at position corresponding to position 58 of SEQ ID NO:36.