IP Library Granted Patent US 11,198,264
Granted Patent B2
US 11,198,264 · App. 14/326,817 · Granted Dec 14, 2021

Dried hydrogel, dried vitrigel membrane, and methods for producing the same

Inventors: Toshiaki Takezawa (Tokyo, JP); Ayumi Oshikata (Ibaraki, JP); Hiroyuki Kuroyama (Kanagawa, JP); Tomoya Sawaguchi (Kanagawa, JP); Hiroyuki Yamaguchi (Kanagawa, JP)
Assignees: KANTO KAGAKU KABUSHIKI KAISHA; Toshiaki Takezawa
B29D7/01B29C39/02B29C41/02C12N5/0062B29K2089/00C12M25/14
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Quick Facts
Patent No.
US 11,198,264
App. No.
14/326,817
Granted
Dec 14, 2021
Kind
B2
Abstract

A dried vitrigel membrane is produced by a method including the following steps of (1) a step of keeping a hydrogel in the inside of a wall surface mold with a shape the same as the desired shape disposed on a substrate, and discharging a part of free water within the hydrogel from a gap between the substrate and the wall surface mold; (2) a step of removing the wall surface mold from the top of the substrate; (3) a step of drying the hydrogel to remove the residual free water, thereby fabricating a vitrified dried hydrogel; (4) a step of rehydrating the dried hydrogel to fabricate a vitrigel membrane; and (5) a step of redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane.

Claims (13)

1. A method for producing a dried vitrigel membrane having a desired shape, comprising the following steps of:

placing a wall surface mold, which is a cylindrical frame body not having a top surface and a bottom surface and having a shape the same as the desired shape, onto a substrate such that a gap is formed between a surface of the substrate and an underside of the cylindrical frame of the wall surface mold;

adding a hydrosol to the inside of the wall surface mold;

forming a hydrogel by gelation of the hydrosol in the inside of the wall surface mold;

allowing a part of a free water within the hydrogel to discharge horizontally through the gap between the surface of the substrate and the underside of the cylindrical frame of the wall surface mold while maintaining the hydrogel in the inside of the wall surface mold;

removing the wall surface mold from the top of the substrate;

drying the hydrogel to remove residual free water, thereby fabricating a vitrified dried hydrogel;

rehydrating the dried hydrogel to fabricate a vitrigel membrane; and

redrying the vitrigel membrane to remove free water, thereby fabricating a vitrified dried vitrigel membrane, which does not have an amorphous outer peripheral edge.

2. The method for producing a dried vitrigel membrane according to claim 1 , wherein in the adding step, a support is introduced into the hydrosol in the inside of the wall surface mold.

3. The method for producing a dried vitrigel membrane according to claim 1 , wherein in the allowing step, an amount of the free water within the hydrogel is reduced to from about ¼ to ¾.

4. The method for producing a dried vitrigel membrane according to claim 1 , wherein in the redrying step, the dried vitrigel membrane is vitrified on a film possessing a capacity facilitating the detachability of the dried vitrigel membrane.

5. The method for producing a dried vitrigel membrane according to claim 4 , wherein the film is Parafilm.

Assignments (3)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 25, 2021
From: NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
To: KANTO KAGAKU KABUSHIKI KAISHA
Reel/Frame 057899/0234 →
CORRECTIVE ASSIGNMENT TO CORRECT THE NEWLY MERGER NAME PREVIOUSLY RECORDED AT REEL: 57435 FRAME: 889. ASSIGNOR(S) HEREBY CONFIRMS THE ASSINGMNET. Recorded Sep 17, 2021
From: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES
To: NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZATION
Reel/Frame 057623/0260 →
MERGER AND CHANGE OF NAME Recorded Sep 7, 2021
From: NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES; NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZAION
To: NATIONAL AGRICULTURE AND FOOD RESEARCH ORGANIZAION
Reel/Frame 057435/0889 →
Priority Claims (1)
JP JP2010-188887 · Aug 25, 2010 · national
Continuity (2)
Division 13818759
Related Publication 20140319728A1 · Oct 30, 2014