Methods, systems, and arrays for biomolecular analysis
Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.
1. A method for obtaining feature binding data, comprising:
contacting a microarray with a sample comprising a plurality of ligands at a concentration that is less than or equal to 10 μg/ml under conditions that promote ligand binding, wherein said sample has a volume that is less than or equal to 25 μl; and
wherein said microarray comprises at least 100,000 features per square centimeter, and wherein said microarray has an area that is less than or equal to 100 square millimeters wherein each of the features comprises a collection of peptides wherein said collection of peptides is characterized by an average length of at least five coupled amino acids and characterized by an average coupling efficiency for each amino acid coupling step of at least 98.5%; and
imaging said microarray with an imaging device to identify binding of said plurality of ligands to said features of said microarray, wherein a coefficient of variation of data obtained from said microarray is not greater than 5 percent and wherein said imaging comprises identifying binding of at least 100,000 ligands to said features of said microarray.
2. The method of claim 1 , wherein an elapsed time from sample contacting to finishing the imaging is less than 20 minutes.
3. The method of claim 1 , wherein an elapsed time for imaging is equal or less than 20 seconds.
4. The method of claim 1 , wherein said microarray comprises at least 1,000,000 features per square centimeter.
5. The method of claim 4 , wherein said microarray comprises at least 15,000,000 features per square centimeter.