Fluidic connectors and microfluidic systems
Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use.
1. A method comprising:
in a fluidic device containing a first fluid, wherein the first fluid is stored in a region of the device that includes a fluid-tight seal, performing the steps of:
unsealing the fluid-tight seal;
introducing a sample into the fluidic device after the unsealing step by inserting a sample introduction component containing the sample into a port of the fluidic device, wherein the step of inserting the sample introduction component containing the sample into the port of the fluidic device causes the unsealing of the fluid-tight seal;
passing the sample across a reaction area of the fluidic device;
passing the first fluid across the reaction area; and
determining a component of the sample at the reaction area.
2. The method of claim 1 , wherein the sample introduction component remains attached to the fluidic device during the steps of passing the sample and the first fluid across the reaction area.
3. The method of claim 1 , wherein the fluid-tight seal is a cover on a port of the fluidic device.
4. The method of claim 3 , wherein inserting the sample introduction component containing the sample into the port of the fluidic device causes the cover of the port to be pierced or broken.
5. The method of claim 1 , wherein the step of introducing the sample into the fluidic device comprises applying a vacuum to an outlet of the fluidic device to cause the sample to flow from the sample introduction component into the fluidic device.
6. The method of claim 1 , comprising passing the first fluid across the reaction area after the step of passing the sample across the reaction area.
7. The method of claim 1 , wherein the fluid-tight seal is a piercable seal.
8. The method of claim 1 , wherein the first fluid is stored in a microfluidic channel having at least one cross-sectional dimension of less than 1 mm.
9. The method of claim 1 , wherein the first fluid is a wash fluid.
10. The method of claim 1 , wherein the sample introduction component comprises an inlet and an outlet, the method comprising inserting both the inlet and outlet into ports of the fluidic device.
11. The method of claim 1 , wherein the sample introduction component, when inserted into the port to the fluidic device, is downstream of the region containing the first fluid and upstream of the reaction area.