Tissue regeneration and wound treatment methods with platelet derived compositions
This invention provides methods for extracting platelets, compositions obtained therefore, and methods for using the same.
1. A method for regenerating a tissue or treating a wound in a subject, said method comprising contacting a desired site for tissue regeneration or the wound in a subject with a composition comprising a growth factor, wherein said composition is produced by a process comprising:
disrupting pelletized platelets in an extraction solution to produce an insoluble material portion and the extraction solution comprising a growth factor; and
removing insoluble materials and proteins having molecular weight of 2 kD or less from said extraction solution to produce a composition comprising a growth factor.
2. The method of claim 1 , wherein said process further comprises subjecting said extraction solution to an ultrafiltration process to remove any microorganisms that maybe present.
3. The method of claim 2 , wherein said process further comprises lyophilizing said extraction solution to produce a solid composition comprising the growth factor.
4. The method of claim 3 , wherein said process further comprises dissolving said solid composition in a solution to produce a fluid composition comprising the growth factor.
5. The method of claim 1 , wherein said growth factor comprises VEGF, TGF-β1, TGF-β2, PDGF-BB, EGF, or a mixture thereof.
6. The method of claim 1 , wherein the desired site for tissue regeneration comprises a burn site, a graft donor site, an oral and maxillofacial surgery site, a mucogingival surgery site, a perioplastic surgery site, an oral mucosa site, or a combination thereof.
7. A method for reducing a risk of microbial infection in a wound area of a subject, said method comprising treating the wound area of a subject with a wound treatment composition produced by a process comprising:
disrupting pelletized platelets in a solution to produce an insoluble material portion and an extraction solution; and
removing insoluble materials and proteins having molecular weight of 2 kD or less from said extraction solution to produce a wound treatment composition.
8. The method of claim 7 , wherein said process further comprises subjecting said extraction solution to an ultrafiltration process to remove any microorganisms that maybe present.
9. The method of claim 8 , wherein said process further comprises lyophilizing said extraction solution to produce a solid wound treatment composition.
10. The method of claim 9 , wherein said process further comprises dissolving said solid wound treatment composition in a solution to produce a solution of wound treatment composition.