Synbiotic mixture
View Patent ↗This invention relates to a preparation comprising a probiotic bacterial strain and a prebiotic mixture comprising 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of Ga1NAcα1-3Ga1β1-4G1c, Ga1β1-6Ga1Nacα1-3Ga1β1-4G1c and combinations thereof, 20-95 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and 2-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Ga1β1-4G1c and combinations thereof. The invention extends to food products comprising said preparation and to the use of the preparation in the prevention and treatment of infections.
1. A method for treatment of an immune condition comprising the step of administering to an individual having the immune condition a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of Galactose(“Gal”)-N-acetyl(“NAc”)α1-3Galβ1-4Glucose(“Glc”), Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of Neuraminic acid(“Neu”)Acα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.
2. The method of claim 1 , wherein the oligosaccharide mixture comprises 10-70 wt % of the at least one N-acetylated oligosaccharide, 20-80 wt % of the at least one neutral oligosaccharide and 10-50 wt % of the at least one sialylated oligosaccharide.
3. The method of claim 1 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.
4. The method of claim 1 , wherein the immune condition is selected from the group consisting of food hypersensitivity, eczema, allergic rhinitis, atopic dermatitis, and combinations thereof.
5. A method for treatment of an infection of the upper respiratory tract comprising the step of administering to an individual having the infection of the upper respiratory tract a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.
6. The method of claim 5 , wherein the oligosaccharide mixture comprises 10-70 wt % of the at least one N-acetylated oligosaccharide, 20-80 wt % of the at least one neutral oligosaccharide and 10-50 wt % of the at least one sialylated oligosaccharide.
7. The method of claim 5 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.
8. The method of claim 5 , wherein the infection of the upper respiratory tract is otitis media.
9. A method for establishing a bifidogenic intestinal microbiota and/or increasing the metabolic activity of such bifidogenic intestinal microbiota comprising the steps of administering to an individual in need thereof a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.
10. The method of claim 9 , wherein the individual is an infant.
11. A method for treatment of a pathogenic infection of the gastro-intestinal tract comprising the step of administering to an individual having the pathogenic infection of the gastro-intestinal tract a composition comprising a probiotic bacterial strain and a prebiotic mixture comprising
a) 5-70 wt % of at least one N-acetylated oligosaccharide selected from the group consisting of GalNAcα1-3Galβ1-4Glc, Galβ1-6GalNacα1-3Galβ1-4Glc and combinations thereof,
b) 20-90 wt % of at least one neutral oligosaccharide selected from the group consisting of Galβ1-6Gal, Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-6Glc, Galβ1-3Galβ1-3Glc, Galβ1-3Galβ1-4Glc, Galβ1-6Galβ1-6Galβ1-4Glc, Galβ1-6Galβ1-3Galβ1-4Glc, Galβ1-3Galβ1-6Galβ1-4Glc, Galβ1-3Galβ1-3Galβ1-4Glc and combinations thereof and
c) 5-50 wt % of at least one sialylated oligosaccharide selected from the group consisting of NeuAcα2-3Galβ1-4Glc, NeuAcα2-6Galβ1-4Glc and combinations thereof.
12. The method of claim 11 , wherein the pathogenic infection is caused by at least one pathogen selected from the group consisting of Staphylococci and Clostridium perfringens.
13. The method of claim 11 , wherein the prebiotic mixture comprises 15-40 wt % of the at least one N-acetylated oligosaccharide, 40-60 wt % of the at least one neutral oligosaccharide and 15-30 wt % of the at least one sialylated oligosaccharide.
14. The method of claim 11 wherein the probiotic bacterial strain is a Lactobacillus strain.
15. The method of claim 14 , wherein the Lactobacillus strain is selected from the group consisting of a Lactobacillus rhamnosus , a Lactobacillus paracasei and a Lactobacillus reuteri.
16. The method of claim 14 , wherein the Lactobacillus strain is Lactobacillus rhamnosus CGMCC 1.3724.
17. The method of claim 14 , wherein the Lactobacillus strain is Lactobacillus paracasei CNCM I-2116.
18. The method of claim 11 , wherein the probiotic bacterial strain is a Bifidobacterium strain.
19. The method of claim 18 , wherein the Bifidobacterium strain is selected from the group consisting of a Bifidobacterium lactis , a Bifidobacterium longum and a Bifidobacterium breve.
20. The method of claim 18 , wherein the Bifidobacterium strain is Bifidobacterium longum ATCC BAA-999.