Peptide array quality control
View Patent ↗The present application provides arrays for use in immunosignaturing and quality control of such arrays. Also disclosed are peptide arrays and uses thereof for diagnostics, therapeutics and research.
1. A method for determining quality and fidelity of an in situ synthesized immunosignature array, comprising:
obtaining a first binding pattern of the in situ synthesized immunosignature array, wherein the first binding pattern is obtained from binding with a first sample, the first sample comprising a biological sample from a subject;
obtaining a second binding pattern of a immunosignature reference array;
comparing the first binding pattern with the second binding pattern and calculating a correlation value, wherein the comparing comprises a direct peptide-by-peptide comparison between the first and the second binding patterns to evaluate a number of peptides expressing different patterns and a comparison between a binding profile of the first binding pattern and a binding profile of the second binding pattern to evaluate a level of similarity between the binding profiles, wherein each of the binding profiles comprises a distribution of signals measured from a population of at least 2 arrayed compounds; and thereby establishing synthesis quality and fidelity of the arrayed immunosignature, wherein a correlation value of less than a threshold level indicates a quality of the immunosignature array.
2. The method of claim 1 , wherein the correlation value is obtained by Kolmogorov-Smimov test, Pearson Correlation or Pearson Squared.
3. The method of claim 1 , wherein the threshold level is defined as a percentage of the correlation coefficient between assays.
4. The method of claim 1 , wherein the immunosignature array is a random or partially random peptide array.
5. The method of claim 1 , wherein the first binding pattern is obtained from a subset of peptides on the in situ synthesized immunosignature array, and the second binding pattern is obtained from a corresponding subset of peptides on the immunosignature reference array.
6. The method of claim 1 , wherein the first binding pattern is obtained from binding with the first sample, the second binding pattern is obtained from binding with a second sample, wherein the first sample and the second sample are from a same species.
7. The method of claim 6 , wherein the first sample and the second sample are not from the same subject.
8. The method of claim 7 , wherein the species is human being.
9. The method of claim 7 , wherein the first sample and the second sample are acquired from a blood sample.
10. The method of claim 1 , wherein the biological sample is a blood sample, a serum sample, a plasma sample, a urine sample, a sputum sample, a mucosal sample, a peritoneal fluid sample, a tissue sample, an exudate or an effusion.