Highly active silica magnetic nanoparticles for purifying biomaterial and preparation method thereof
The present invention relates to a method for preparing highly active silica magnetic nanoparticles, highly active silica magnetic nanoparticles prepared by the method, and a method of isolating nucleic acid using the highly active silica magnetic nanoparticles. The highly active silica magnetic nanoparticles prepared according to the present invention contain magnetic nanoparticles completely coated with silica, can be used as a reagent for isolating biomaterials, particularly, nucleic acids, and can isolate and purify nucleic acid in a high yield.
1. A method for preparing silica-coated magnetic nanoparticles, consisting of:
(a) ultrasonically dispersing a solution consisting of magnetic nanoparticles, an acid catalyst or base catalyst, a hydrophobic organic solvent, water, and an alcohol; and
(b) adding silane to the dispersed solution to prepare silica magnetic nanoparticles,
wherein the silane is added in an amount of 0.01-0.5 parts by volume, based on 100 parts by volume of a total volume of the water, the alcohol, the acid catalyst or the base catalyst, and the hydrophobic organic solvent;
the magnetic nanoparticles are added in an amount of 0.01-0.5 parts by weight, based on 100 parts by weight of a total weight of the water, the alcohol, the acid catalyst or the base catalyst, and the hydrophobic organic solvent;
the magnetic nanoparticles and the silane are added at a weight ratio of 1:0.1-1:3.0.
the silica-coated magnetic nanoparticles have a silica content of 0.5-2.5 wt %, a particle size of 300-600 nm, and a coating thickness of 1-15 nm, and surface of the silica has a hydroxyl group thereon;
the hydrophobic organic solvent is selected from the group consisting of pentane, cyclopentane, hexane, cyclohexane, toluene, benzene, xylene, diethyl ether, dioxane, chloroform, and dichloromethane;
the alcohol is selected from the group consisting of methyl alcohol, ethyl alcohol, propyl alcohol, and butyl alcohol;
the silane is tetramethoxysilane or tetraethoxysilane;
the acid catalyst or base catalyst is selected from the group consisting of ammonia water, ammonium fluoride, hydrochloric acid, nitric acid, sulfuric acid, phosphoric acid, fluoric acid, oxalic acid, and acetic acid; and
the magnetic nanoparticles are one or more selected from the group consisting of iron, cobalt, nickel, iron oxides, cobalt oxides, nickel oxides, and mixtures thereof.
2. The method of claim 1 , wherein the solution contains 30 vol % or less of water, based on total volume of water and alcohol.
3. The method of claim 1 , wherein a thickness of silica coated on a surface of the silica magnetic nanoparticles is controlled according to an added amount of silane.
4. The method of claim 1 , wherein the magnetic nanoparticles are one or more selected from the group consisting of iron oxide, ferrite, and mixtures thereof.
5. The method of claim 1 , wherein:
the magnetic nanoparticles are iron oxide magnetic nanoparticles;
the acid catalyst or base catalyst is ammonia;
the hydrophobic organic solvent is toluene;
the water is ultrapure water;
the alcohol is ethanol; and
the silane is tetraethoxysilane.