Highly sensitive system and method for analysis of troponin
The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.
1. A method for determining an absence of coronary artery disease (CAD) in a subject, the method comprising:
(a) measuring the concentration of at least one of Troponin I (cTnI) and cardiac troponin T (cTnT) in a blood, serum or plasma sample from the subject in an assay comprising counting individual digital events representing binding of a binding partner the at least one of cTnI and cTnT, wherein each individual digital event comprises a signal representing a single molecule of the cTnI and cTnT in the sample, determining a total signal as a sum of the individual digital events, and relating the sum to the concentration of the at least one of cTnI and cTnT in the sample,
(b) determining an absence of CAD in the subject when the concentration of the at least one of cTnI and cTnT in the sample is less than about 10 pg/mL.
2. The method of claim 1 , wherein the subject has a symptom of coronary artery disease.
3. The method of claim 2 , wherein the symptom is at least one of chest pain, shortness of breath, arm pain, abnormal EKG, and abnormal enzyme levels.
4. The method of claim 1 , wherein the CAD is selected from at least one of acute myocardial infarction, cardiac damage, cardiac toxicity, acute coronary syndrome, myocarditis, and heart failure.
5. The method of claim 1 , wherein the absence of CAD is determined when the at least one of cTnI and cTnT in the sample is less than about 9 pg/mL.
6. The method of claim 1 , wherein the absence of CAD is determined when the at least one of cTnI and cTnT in the sample is less than about 8 pg/mL.
7. The method of claim 1 , wherein the absence of CAD is determined when the at least one of cTnI and cTnT in the sample is less than about 7 pg/mL.
8. The method of claim 1 , wherein the sample is taken during a stress test.
9. The method of claim 1 , wherein the sample is taken after a stress test.
10. The method of claim 1 , wherein the assay has a limit of detection for the at least one of cTnI and cTnT of less than 3 pg/mL and a coefficient of variation of less than 10%.
11. The method of claim 1 , wherein the binding partner comprises an antibody specific for the at least one of cTnI and cTnT.
12. The method of claim 11 , wherein the assay detects a photon emission from a label corresponding to a single molecule of the at least one of cTnI and cTnT in the sample, wherein the antibody is bound to or was bound to a molecule of the at least one of cTnI and cTnT in the sample.
13. The method of claim 12 , wherein the antibody is eluted from the at least one of cTnI or cTnT before the photon emission is detected.
14. The method of claim 13 , wherein the photon emission is detected in a single molecule analyzer.
15. The method of claim 1 , wherein the assay is conducted using an analyzer comprising:
(a) an electromagnetic radiation source for stimulating a fluorescent moiety; and
(b) an electromagnetic radiation detector that measures an electromagnetic characteristic of the fluorescent moiety when stimulated by the electromagnetic radiation.
16. The method of claim 1 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
17. The method of claim 1 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnT.
18. A method for determining an absence of coronary artery disease (CAD) in a subject, comprising:
(a) measuring the concentration of at least one of Troponin I (cTnI) and cardiac troponin T (cTnT) in a series of blood, serum or plasma samples from the subject in an assay comprising counting individual digital events representing binding of a binding partner to the at least one of cTnI and cTnT, wherein each individual digital event comprises a signal representing a single molecule of the cTnI and cTnT in the sample, determining a total signal as a sum of the individual digital events, and relating the sum to the concentration of the at least one of cTnI and cTnT in the sample,
(b) determining at least one of a change and a rate of change in the concentration of the at least one of cTnI and cTnT in the series of samples,
(c) determining the absence of coronary artery disease when the at least one of the change and the rate of change of the at least one of cTnI and cTnT in the series of samples is less than a predetermined reference change in concentration of less than 1-10 pg/mL or a predetermined reference rate of change in concentration of 2-10 pg/mL.
19. The method of claim 18 , wherein the assay has a limit of detection of less than 3 pg/mL for the at least one of cTnI and cTnT.
20. The method of claim 18 , wherein the coronary artery disease is at least one of acute myocardial infarction, cardiac damage, cardiac toxicity, acute coronary syndrome, myocarditis, and heart failure.
21. The method of claim 18 , wherein the subject has a symptom of coronary artery disease.
22. The method of claim 21 , wherein the symptom is at least one of abnormal EKG, abnormal enzyme levels, chest pain, arm pain, and shortness of breath.
23. The method of claim 18 , further comprising comparing the concentration of the at least one of cTnI and cTnT to a threshold concentration representing the 99th percentile concentration of the at least of one cTnI and cTnT in a group of normal subjects.
24. The method of claim 23 , wherein the threshold concentration is less than 10 pg/mL.
25. The method of claim 23 , wherein the threshold concentration is less than about 7 pg/ml.
26. The method of claim 23 , wherein the threshold concentration is less than about 8 pg/ml.
27. The method of claim 23 , wherein the threshold concentration is less than about 9 pg/ml.
28. The method of claim 23 , wherein the threshold concentration is within a range of 1 pg/ml to 10 pg/ml.
29. The method of claim 18 , wherein at least one sample of the series of samples is taken during or after a cardiac stress test.
30. The method of claim 18 , wherein the series of samples is taken over a period of up to about 30 hours.
31. The method of claim 30 , wherein the series of samples is taken at intervals of less than about 4 hours.
32. The method of claim 18 , wherein the binding partner comprises an antibody specific for the at least one of cTnI and cTnT.
33. The method of claim 32 , wherein the assay detects a photon emission from a label corresponding to a single molecule of the at least one of cTnI and cTnT in the sample, wherein the antibody is bound to or was bound to a molecule of the at least one of cTnI and cTnT in the sample.
34. The method of claim 33 , wherein the antibody is eluted from the at least one of cTnI or cTnT before the photon event.
35. The method of claim 33 , wherein the photon emission is detected in a single molecule analyzer.
36. The method of claim 18 , wherein the assay is conducted using an analyzer comprising:
(a) an electromagnetic radiation source for stimulating a fluorescent moiety; and
(b) an electromagnetic radiation detector that measures an electromagnetic characteristic of the fluorescent moiety when stimulated by the electromagnetic radiation.
37. The method of claim 18 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
38. The method of claim 18 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnT.
39. The method of claim 1 , wherein the assay has a sensitivity for the at least one of cTnI and cTnT of less than 3 pg/mL.
40. The method of claim 1 , wherein the assay has a sensitivity for the at least one of cTnI and cTnT of less than 1 pg/mL.
41. The method of claim 18 , wherein the assay has a sensitivity for the at least one of cTnI and cTnT of less than 3 pg/mL.
42. The method of claim 18 , wherein the assay has a sensitivity for the at least one of cTnI and cTnT of less than 1 pg/mL.
43. The method of claim 39 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
44. The method of claim 40 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
45. The method of claim 41 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
46. The method of claim 42 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
47. The method of claim 2 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
48. The method of claim 5 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
49. The method of claim 6 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
50. The method of claim 7 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
51. The method of claim 10 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
52. The method of claim 12 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
53. The method of claim 19 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
54. The method of claim 23 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
55. The method of claim 24 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
56. The method of claim 25 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
57. The method of claim 26 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
58. The method of claim 27 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.
59. The method of claim 28 , wherein the at least one of Cardiac Troponin I (cTnI) and cardiac troponin T (cTnT) is cTnI.