Bacillus bacteria for use in treating and preventing infection in aquatic animals
Disclosed herein are microbiocidal compositions that include spore-forming strains of Bacillus . The compositions may be utilized to treat or prevent disease in aquatic animals such as farmed fish or crustaceans.
1. A probiotic-amended feed composition formulated for feeding aquatic animals, the composition formulated by adding a Bacillus to a feed composition for aquatic animals, the Bacillus added at a concentration of at least 10 4 CFU/g of the feed composition, wherein the Bacillus is a spore-forming strain of Bacillus having a 16S rDNA sequence comprising SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3 or comprising a 16S rDNA sequence having at least 99% sequence identity to SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3.
2. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus is a Bacillus species having the 16S rDNA sequence comprising SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3.
3. The feed composition of claim 1 , wherein the feed composition is a feed composition for farmed fish.
4. The feed composition of claim 1 , comprising a single strain of the Bacillus.
5. The feed composition of claim 1 , comprising a mixture of the spore-forming strains of the Bacillus.
6. The feed composition of claim 1 , wherein the spore-forming strain of the Bacillus inhibits the growth of one or more bacteria selected from the group consisting of Aeromonas hydrophila, Edwardsiella ictaluri, Edwardsiella tarda, Flavobacterium columnare, Streptococcus iniae , and Yersinia ruckeri.
7. The feed composition of claim 1 , further comprising an agent selected from the group consisting of sulfadimethoxine, ormetoprim, and florfenical.
8. A probiotic-amended feed composition formulated by adding a Bacillus to a feed composition for aquatic animals, the Bacillus added at a concentration of at least 10 4 CFU/g of the feed composition, wherein the Bacillus is a spore-forming strain of Bacillus selected from the group consisting of strains deposited under accession numbers NRRL B-50741, NRRL B-50742, NRRL B-50743, NRRL B-50744, and NRRL B-50745.
9. The feed composition of claim 8 , wherein the feed composition is a feed composition for farmed fish.
10. The feed composition of claim 8 , comprising a single strain of the Bacillus.
11. The feed composition of claim 8 , comprising a mixture of the spore-forming strains of the Bacillus.
12. The feed composition of claim 8 , wherein the spore-forming strain of the Bacillus inhibits the growth of one or more bacteria selected from the group consisting of Aeromonas hydrophila, Edwardsiella ictaluri, Edwardsiella tarda, Flavobacterium columnare, Streptococcus iniae , and Yersinia ruckeri.
13. The feed composition of claim 8 , further comprising an agent selected from the group consisting of sulfadimethoxine, ormetoprim, and florfenical.