Methods and compositions for targeting progenitor cell lines
The invention provides methods, compositions and kits for the identification and enrichment of progenitor cell lines obtained from pluripotent stem cells.
1. A method of enriching a population of progenitor cells, wherein the progenitor cells are the in vitro progeny of pluripotent stem cells, comprising contacting a population of cells comprising a progenitor cell with a peptide that binds specifically to the progenitor cell, wherein the peptide is chosen from SWTYSYPNQNMD (SEQ ID NO: 1); DWTYSLPGLVEE (SEQ ID NO: 2); NWTWSMPTGNPA (SEQ ID NO: 3); GMTLRVLTN-YTE- (SEQ ID NO: 4); TLHVSENSWTYN (SEQ ID NO: 5); DWLWSFAPNVDT (SEQ ID NO: 6); TLSSQNPYMHKK (SEQ ID NO: 7); IDKQMMTSHKAI (SEQ ID NO: 8); QGMETQKLRMLK (SEQ ID NO: 9); GWYWETPLDMFN (SEQ ID NO: 10); GWVIDYDYYPMR (SEQ ID NO: 11); VTAENYQSFSVS (SEQ ID NO: 12); NNKMDDRMMMSIV (SEQ ID NO: 13); STGTDLHSNARI (SEQ ID NO: 14); YEFDNLLNRTLW (SEQ ID NO: 15); EWTVNERTMWDL (SEQ ID NO: 16), and separating the progenitor cell bound to the peptide from the population of cells thereby enriching for a population of progenitor cells.
2. The method of claim 1 , wherein the progenitor cell is a human cell.
3. The method of claim 2 wherein the progenitor cell is an endoderm cell.
4. The method of claim 2 , wherein the progenitor cell is a mesoderm cell.
5. The method of claim 2 , wherein the progenitor cell is a W10 progenitor cell.
6. The method of claim 1 , wherein the progenitor cell expresses the markers heart and neural crest derivatives-expressed 2 (HAND2), HOXA4 and HOXB7, and wherein the peptide binds specifically to the progenitor cell expressing HAND2, HOXA4 and HOXB7 proteins.
7. The method of claim 1 , wherein the peptide has plexin homology.