Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A method for degrading or converting a cellulosic material, said method comprising:
(a) treating the cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having cellobiohydrolase activity; and
(b) recovering the degraded or converted cellulosic material;
wherein the polypeptide having cellobiohydrolase activity has at least 95% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
2. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
3. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 98% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
4. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
5. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most ten amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
6. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most five amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
7. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most three amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
8. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by at most two amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
9. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity differs by one amino acid from the sequence of amino acids 19-469 of SEQ ID NO: 2.
10. The method of claim 1 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 19-469 of SEQ ID NO: 2.
11. A method for producing a fermentation product, said method comprising:
(a) saccharifying a cellulosic material with an enzyme composition, wherein the composition comprises a polypeptide having cellobiohydrolase activity;
(b) fermenting the saccharified cellulosic material with one or more fermenting microorganisms to produce the fermentation product; and
(c) recovering the fermentation product from the fermentation;
wherein the polypeptide having cellobiohydrolase activity has at least 95% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
12. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 97% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
13. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 98% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
14. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity has at least 99% sequence identity to the sequence of amino acids 19-469 of SEQ ID NO: 2.
15. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity differs by at most ten amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
16. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity differs by at most five amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
17. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity differs by at most three amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
18. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity differs by at most two amino acids from the sequence of amino acids 19-469 of SEQ ID NO: 2.
19. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity differs by one amino acid from the sequence of amino acids 19-469 of SEQ ID NO: 2.
20. The method of claim 11 , wherein the polypeptide having cellobiohydrolase activity comprises the sequence of amino acids 19-469 of SEQ ID NO: 2.