Apparatuses and methods for generating and measuring environmental levels of allergens
The present disclosure provides an apparatus comprising an air circulating device, an allergen source comprising an allergen, and a housing. The housing can be collapsible, portable, disposable and configurable. The present disclosure also provides a method comprising positioning an allergen source comprising an allergen in a chamber, distributing at least a portion of the allergen from the allergen source within the chamber, and collecting at least a portion of the distributed allergen. In an embodiment, the level of the shed allergen can be used to measure the effectiveness of various measures implemented to reduce airborne Fel d1 that is emitted from soiled cat litter. In another embodiment, the allergen can be introduced at a desired level in the chamber, and the resultant symptoms of a subject (e.g. a human) within the chamber can be identified and correlated with the amount of allergen.
1. A method comprising:
positioning a first allergen source comprising an allergen in a chamber;
distributing at least a portion of the allergen from the first allergen source within the chamber; and
collecting at least a portion of the distributed allergen;
wherein the chamber is in a first configuration during the positioning of the allergen source, the distributing of the allergen, and the collecting of the allergen, and the method further comprises arranging the chamber in a second configuration that has a size smaller than the first configuration.
2. The method of claim 1 , wherein the allergen is Feline domesticus allergen number 1.
3. The method of claim 1 , further comprising measuring the amount of the collected allergen by performing a Fel d1 enzyme-linked immunosorbent assay (ELISA) on the collected allergen.
4. The method of claim 1 , wherein an individual is present in the chamber during at least a portion of the distributing of the allergen, and the method further comprises measuring symptoms of the individual.
5. The method of claim 1 , wherein the distributing of the allergen comprises maintaining at least a portion of the allergen in suspension in the chamber.
6. The method of claim 1 , wherein the distributing of the allergen comprises circulating air in the chamber using a first fan.
7. The method of claim 6 , wherein the distributing of the allergen comprises circulating air in the chamber using a second fan positioned on an opposite side of the allergen source relative to the first fan.
8. The method of claim 7 , wherein the second fan is an oscillating fan.
9. The method of claim 1 , wherein the distributing of the allergen is performed for a predetermined time period.
10. The method of claim 1 , wherein the allergen source comprises a predetermined amount of the allergen when positioned in the chamber.
11. A method comprising:
performing a first test comprising positioning a first allergen source comprising a first amount of an allergen in a first chamber, distributing at least a portion of the first amount of the allergen within the first chamber by circulating air in the first chamber using a first fan and a second fan, the second fan be positioned on an opposite side of the first allergen source relative to the first fan, collecting at least a portion of the distributed allergen as a first sample, and measuring the amount of the collected allergen by performing a Fel d1 enzyme-linked immunosorbent assay (ELISA) on the first sample; and
performing a second test comprising positioning a second allergen source comprising a second amount of the allergen in a second chamber, distributing at least a portion of the second amount of the allergen within the second chamber by circulating air in the second chamber using a first fan and a second fan, the second fan be positioned on an opposite side of the second allergen source relative to the first fan, collecting at least a portion of the distributed allergen as a second sample, and measuring the amount of the collected allergen by performing the Fel d1 enzyme-linked immunosorbent assay (ELISA) on the second sample.
12. The method of claim 11 , wherein the first chamber and the second chamber are the same chamber, and the method further comprises cleaning a non-porous flexible shell of the chamber between the first test and the second test.
13. The method of claim 11 , wherein the first chamber and the second chamber are different chambers, and the first chamber and the second chamber are positioned next to each other while the first and second tests are contemporaneously performed.
14. A method of quantitatively determining a cat litter or a diet that produces lower emission of an allergen to an environment, the method comprising:
performing a first test comprising positioning a first allergen source comprising a first amount of the allergen in a first chamber, distributing at least a portion of the first amount of the allergen within the first chamber, and collecting at least a portion of the distributed allergen as a first sample;
measuring the amount of the allergen in the first sample;
performing a second test comprising positioning a second allergen source comprising a second amount of the allergen in a second chamber, distributing at least a portion of the second amount of the allergen within the second chamber, and collecting at least a portion of the distributed allergen as a second sample;
measuring the amount of the allergen in the second sample; and
comparing the amount of the allergen in the first sample to the amount of the allergen in the second sample to determine whether the first sample or the second sample has a lower allergen content;
wherein the first allergen source is a first cat litter, a first hair sample from a cat, or first fecal matter from the cat having been fed a first diet and wherein the second allergen source is a second cat litter, a second hair sample from the cat, or a second fecal matter from the cat having been fed a second diet.