IP Library › Granted Patent US 9,745,550
Granted Patent B2
US 9,745,550 · App. 15/005,397 · Granted Aug 29, 2017

Synthetic surfaces for culturing stem cell derived cardiomyocytes

Inventors: Christopher Bankole Shogbon (Corning, NY); Yue Zhou (Horseheads, NY); Ralph Brandenberger (Palo Alto, CA)
Assignee: Asterias Biotherapeutics, Inc.
C12N5/0657C12N5/0068C12N2500/90C12N2506/02C12N2533/30
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Quick Facts
Patent No.
US 9,745,550
App. No.
15/005,397
Granted
Aug 29, 2017
Kind
B2
Abstract

Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.

Claims (7)

1. A method for culturing pluripotent stem-cell derived cardiomyocytes, comprising: depositing a suspension comprising the pluripotent stem-cell derived cardiomyocytes on a polymer material, and culturing the deposited pluripotent stem-cell derived cardiomyocytes in a cell culture medium, wherein the polymer material comprises a diacrylate or a dimethacrylate.

2. The method of claim 1 , wherein the diacrylate is poly(ethylene glycol) diacrylate.

3. The method of claim 1 , wherein the dimethacrylate is glycerol dimethacrylate.

4. The method of claim 1 , wherein the dimethacrylate is 1,4-butanediol dimethacrylate.

5. The method of claim 1 wherein the pluripotent stem-cell derived cardiomyocytes are human cells.

6. The method of claim 1 , wherein the pluripotent stem-cell derived cardiomyocytes are human embryonic stem-cell derived cardiomyocytes.

7. The method of claim 1 , wherein the cell culture medium is a chemically defined medium.

Continuity (5)
Continuation 14028808 · Sep 17, 2013
Continuation 13546381 · Jul 11, 2012
Division 12362190 · Jan 29, 2009
Provisional Application 61062921 · Jan 30, 2008
Related Publication 20160137983A1 · May 19, 2016