Generation of glucosamine from plant material
View Patent ↗The invention relates to an efficient and reproducible process for generating glucosamine from plants which allows high glucosamine content to be obtained while reducing the content of residual ammonium or sulfate ions. The invention also concerns food compositions or cosmetic compositions which comprise the obtained glucosamine-enriched plant compositions.
1. A process for making a food or cosmetic composition, the process comprising the following successive steps:
placing a plant material and at least one glucosamine precursor into a receptacle, wherein the at least one glucosamine precursor is ammonium sulfate and the plant material is selected from the group consisting of root of Cichorium intybus , root of Daucus carota , tuber of Helianthus tuberosum , root of Beta vulgaris , and mixtures thereof; closing the receptacle;
heating the receptacle at a temperature between 70° C. and 110° C. for a time period more than 10 hours to form a glucosamine-enriched plant composition;
extracting the glucosamine-enriched plant composition with a buffer solution having a pH equal to or greater than 10 to form a glucosamine-enriched plant extract;
adjusting a pH of the glucosamine-enriched plant extract to 6.5 to 7, adding a solution of calcium chloride and adding ethanol, to form a precipitate;
separating the precipitate from the glucosamine-enriched plant extract, to form a glucosamine-enriched solution;
isolating dry matter comprising glucosamine from the glucosamine-enriched solution; and
incorporating the dry matter into the food or cosmetic composition.
2. The process according to claim 1 , wherein the time period of the heating is 48 hours to 96 hours.
3. The process according to claim 1 , which further comprises the following steps:
opening the receptacle; and
drying the glucosamine-enriched plant composition.
4. The process according to claim 1 , wherein the temperature of the heating is 75° C. to 91° C.
5. The process according to claim 1 , wherein the buffer solution is a carbonate solution at pH 10.8.
6. The process according to claim 1 , wherein the dry matter comprises less than 5 g of ammonium ions per kg of dry matter.
7. The process according to claim 1 , wherein the dry matter comprises less than 5 g of sulfate ions per kg of dry matter.
8. The process according to claim 1 , wherein the dry matter comprises less than 1 g of ammonium ions per kg of dry matter and less than 2 g of sulfate ions per kg of dry matter.