Systems and methods for quantifying an analyte extracted from a sample
The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.
1. A method for extracting an analyte from a sample, the method comprising:
introducing a solvent into a capillary;
introducing the capillary into a vessel comprising a sample such that a portion of the sample is introduced into the capillary;
moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, wherein the sample and the solvent do not mix with each other;
analyzing the analyte that has been extracted from the sample; and
quantifying the analyte, without knowledge of a volume of the sample and the solvent.
2. The method according to claim 1 , wherein analyzing comprises:
applying a voltage to the solvent comprising the extracted analyte in the capillary so that the analyte is expelled from the capillary, thereby generating ions of the analyte; and
analyzing the ions.
3. The method according to claim 1 , wherein analyzing comprises:
removing the solvent comprising the extracted analyte from the capillary; and
conducting an assay that analyzes the analyte.
4. The method according to claim 3 , wherein the assay comprises:
generating ions of the analyte; and
analyzing the ions.
5. The method according to claim 1 , wherein the solvent comprises an internal standard.
6. The method according to claim 1 , wherein the sample comprises an internal standard.
7. The method according to claim 1 , wherein the solvent is immiscible with the sample.
8. The method according to claim 1 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.