IP Library › Granted Patent US 12,374,538
Granted Patent B2
US 12,374,538 · App. 18/526,244 · Granted Jul 29, 2025

Systems and methods for quantifying an analyte extracted from a sample

Inventors: Zheng Ouyang (West Lafayette, IN); Yue Ren (West Lafayette, IN); Ziqing Lin (West Lafayette, IN)
Assignee: Purdue Research Foundation
H01J49/167C12Q1/6806Y10T436/24Y10T436/255
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Quick Facts
Patent No.
US 12,374,538
App. No.
18/526,244
Granted
Jul 29, 2025
Kind
B2
Abstract

The invention generally relates to systems and methods for quantifying an analyte extracted from a sample. In certain embodiments, the invention provides methods that involve introducing a solvent into a capillary, introducing the capillary into a vessel including a sample such that a portion of the sample is introduced into the capillary, moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent, thereby causing the analyte to be extracted from the sample and into the solvent, analyzing the analyte that has been extracted from the sample, and quantifying the analyte. In certain embodiments, the quantifying step is performed without knowledge of a volume of the sample and/or solvent.

Claims (41)

1. A method for analyzing a reaction, the method comprising:

introducing a solvent into a capillary;

introducing the capillary into a vessel comprising a sample comprising an analyte and a reactant such that a portion of the sample comprising the analyte and the reactant is introduced into the capillary;

allowing the analyte and the reactant to react with each other in the capillary to form a reaction product, which reaction continues over time along with formation of the reaction product over time;

moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent while the sample and the solvent remain separate from each other, thereby causing the reaction product to be extracted from the sample and into the solvent;

analyzing a first amount of formation of the reaction product that has been extracted from the sample at a first time point; and

repeating the moving and analyzing steps at least one more time to analyze at least a second amount of formation of the reaction product at least at a second time point, thereby monitoring reaction product formation over time.

2. The method according to claim 1 , wherein analyzing comprises:

applying a voltage to the solvent comprising the extracted reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and

analyzing the ions.

3. The method according to claim 1 , wherein analyzing comprises:

removing the solvent comprising the extracted reaction product from the capillary; and

conducting an assay that analyzes the reaction product.

4. The method according to claim 3 , wherein the assay comprises:

generating ions of the reaction product; and

analyzing the ions.

5. The method according to claim 1 , wherein the solvent comprises an internal standard.

6. The method according to claim 1 , wherein the sample comprises an internal standard.

7. The method according to claim 1 , wherein the solvent is immiscible with the sample.

8. The method according to claim 1 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

9. A method for analyzing an enzymatic reaction, the method comprising:

introducing a solvent into a capillary;

introducing the capillary into a vessel comprising a sample comprising an enzyme and a reactant such that a portion of the sample comprising the enzyme and the reactant is introduced into the capillary;

allowing the enzyme and the reactant to react with each other in the capillary to form a reaction product, which reaction continues over time along with formation of the reaction product over time;

moving the sample and the solvent within the capillary to induce circulation within the sample and the solvent while the sample and the solvent remain separate from each other, thereby causing the reaction product to be extracted from the sample and into the solvent;

analyzing a first amount of formation of the reaction product that has been extracted from the sample at a first time point; and

repeating the moving and analyzing steps at least one more time to analyze at least a second amount of formation of the reaction product at least at a second time point, thereby monitoring reaction product formation over time.

10. The method according to claim 9 , wherein analyzing comprises:

applying a voltage to the solvent comprising the extracted reaction product in the capillary so that the reaction product is expelled from the capillary, thereby generating ions of the reaction product; and

analyzing the ions.

11. The method according to claim 9 , wherein analyzing comprises:

removing the solvent comprising the extracted reaction product from the capillary; and

conducting an assay that analyzes the reaction product.

12. The method according to claim 11 , wherein the assay comprises:

generating ions of the reaction product; and

analyzing the ions.

13. The method according to claim 9 , wherein the solvent is introduced to the capillary first.

14. The method according to claim 9 , wherein the sample is introduced to the capillary first.

15. The method according to claim 9 , wherein the solvent comprises an internal standard.

16. The method according to claim 9 , wherein the solvent is immiscible with the sample.

17. The method according to claim 9 , wherein the solvent is miscible with the sample and the method further comprises introducing a bridging solvent into the capillary that is immiscible with the solvent and the sample in a manner in which the bridging solvent is between the solvent and the sample.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 1, 2024
From: OUYANG, ZHENG; REN, YUE; LIN, ZIQING
To: PURDUE RESEARCH FOUNDATION
Reel/Frame 066964/0850 →
Continuity (9)
Continuation 17979972 · Nov 3, 2022
Continuation 16985553 · Aug 5, 2020
Continuation 16293017 · Mar 5, 2019
Continuation 15064865 · Mar 9, 2016
Continuation In Part PCTUS2015013649 · Jan 30, 2015
Provisional Application 62130024 · Mar 9, 2015
Provisional Application 62013007 · Jun 17, 2014
Provisional Application 61942949 · Feb 21, 2014
Related Publication 20240162026A1 · May 16, 2024
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