Trialkyl cationic lipids and methods of use thereof
The present invention provides compositions and methods for the delivery of therapeutic agents to cells. In particular, these include novel, trialkyl, cationic lipids and nucleic acid-lipid particles that provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells in vivo. The compositions of the present invention are highly potent, thereby allowing effective knock-down of a specific target protein at relatively low doses.
1. A lipid particle comprising mRNA as a therapeutic agent and a lipid having the structure of Compound 13, or a salt thereof,
wherein the lipid particle comprises about 55 mol % of the lipid having the structure of Compound 13 or a salt thereof, and from about 1.2 mol % to about 1.6 mol % of PEG2000-C-DMA.
2. The lipid particle of claim 1 , wherein the particle further comprises a non-cationic lipid.
3. The lipid particle of claim 2 , wherein the non-cationic lipid is selected from the group consisting of a phospholipid, cholesterol, or a mixture of a phospholipid and cholesterol.
4. The lipid particle of claim 3 , wherein the phospholipid is dipalmitoylphosphatidylcholine (DPPC), di stearoylphosphatidylcholine (DSPC), or a mixture thereof.
5. The lipid particle of claim 1 , which lipid particle comprises from about 1.3 mol % to about 1.6 mol % of PEG2000-C-DMA.
6. The lipid particle of claim 5 , which lipid particle comprises about 1.3 mol % of PEG2000-C-DMA.
7. The lipid particle of claim 5 , which lipid particle comprises about 1.4 mol % of PEG2000-C-DMA.
8. The lipid particle of claim 5 , which lipid particle comprises about 1.5 mol % of PEG2000-C-DMA.
9. The lipid particle of claim 5 , which lipid particle comprises about 1.6 mol % of PEG2000-C-DMA.