Methods for predicting post-operative recurrence of Crohn's disease
The present invention provides methods for predicting post-operative recurrence of Crohn's disease (CD) in a subject. With the present invention it is possible to predict whether a patient undergoing surgical treatment of CD is at risk of developing histological, radiographic, endoscopic, and/or clinical recurrence of the disease.
1. A method for predicting post-operative recurrence of Crohn's disease (CD) in a subject with CD that is undergoing or planning on undergoing surgical treatment of the CD, the method comprising:
(a) determining the presence of a first risk factor by detecting an elevated level of an anti-flagellin antibody in a pre-operative sample from the subject relative to a level of the anti-flagellin antibody from an average subject with CD;
(b) determining the presence of a second risk factor by detecting the presence of perinuclear anti-neutrophilic cytoplasmic antibodies (pANCA) in the sample;
(c) determining the presence of a third risk factor by determining whether the subject is an active smoker;
(d) predicting a risk of post-operative recurrence of CD in the subject based upon the presence of the first, second, and third risk factors, wherein the recurrence of CD comprises endoscopic recurrence, histological recurrence, or clinical recurrence,
wherein the presence of one risk factor is predictive of an increased risk of post-operative recurrence of CD relative to an average subject with CD undergoing surgical treatment of the CD, wherein the presence of two of the risk factors is predictive of a greater risk of post-operative recurrence of CD relative to the presence of one of the risk factors, and wherein the presence of three of the risk factors is predictive of greater risk of post-operative recurrence of CD relative to the presence of two of the risk factors; and
(e) providing a post-operative CD therapy to the subject based on the presence of one two or three of the risk factors.
2. The method of claim 1 , wherein the anti-flagellin antibody is selected from the group consisting of an anti-Fla2 antibody, an anti-FlaX antibody, and an anti-CBir1 antibody.
3. The method of claim 1 , wherein the anti-flagellin antibody comprises an anti-Fla2 antibody.
4. The method of claim 1 , wherein pANCA is positive.
5. The method of claim 1 , wherein the elevated level of the anti-flagellin antibody comprises greater than 66 EU/ml of the anti-flagellin antibody.
6. The method of claim 1 , wherein the post-operative recurrence comprises an endoscopic recurrence score of i3 or i4.
7. The method of claim 1 , wherein the surgical treatment comprises an intestinal resection.
8. The method of claim 1 , wherein the post-operative CD therapy comprises an additional surgical intervention.
9. The method of claim 1 , wherein the post-operative CD therapy comprises a prophylactic drug treatment.
10. A method for predicting post-operative recurrence of CD in a subject with CD that is undergoing or planning on undergoing surgical treatment of the CD, the method comprising:
(a) determining the presence of a first risk factor by detecting, by an immunoassay, a level of an anti-flagellin antibody greater than 66 EU/ml in a pre-operative sample from the subject;
(b) determining the presence of a second risk factor by detecting the presence of pANCA in the sample; and
(c) determining the presence of a third risk factor by determining whether the subject is an active smoker;
(d) predicting a risk of post-operative recurrence of CD in the subject based upon the presence of the first, second, and third risk factors, wherein the recurrence of CD comprises endoscopic recurrence, histological recurrence, or clinical recurrence,
wherein the presence of one risk factor is predictive of an increased risk of post-operative recurrence of CD relative to an average subject with CD undergoing surgical treatment of the CD, wherein the presence of two of the risk factors is predictive of a greater risk of post-operative recurrence of CD relative to the presence of one of the risk factors, and wherein the presence of three of the risk factors is predictive of greater risk of post-operative recurrence of CD relative to the presence of two of the risk factors.
11. The method of claim 10 , further comprising: (e) providing a post-operative CD therapy to the subject based on the presence of one, two, or three of the risk factors.
12. The method of claim 10 , wherein the post-operative recurrence comprises endoscopic recurrence.
13. The method of claim 10 , wherein the surgical treatment comprises an intestinal resection.
14. The method of claim 10 , wherein the anti-flagellin antibody comprises an anti-Fla2 antibody.
15. The method of claim 1 , wherein the sample comprises a serum sample.
16. The method of claim 10 , wherein the sample comprises a serum sample.