Injectable monophase hydrogels
An injectable monophase hydrogel is provided and is made of a reaction mixture of a high molecular weight hyaluronic acid and a low molecular weight hyaluronic acid.
1. An injectable composition prepared by a process comprising the steps of:
providing a first hyaluronic acid salt, having a molecular weight in the range of about 1.1×10 6 Da to about 5×10 6 Da;
providing a second hyaluronic acid salt, having a molecular weight in the range of about 1×10 4 Da to about 9.9·10 5 Da;
mixing the first hyaluronic acid salt and second hyaluronic acid salt to obtain a reaction mixture having an intrinsic viscosity of less than 1900 ml/g, wherein the reaction mixture comprises about 5% by weight to about 30% by weight of the first hyaluronic acid salt;
cross-linking the mixture in an aqueous solvent in the presence of an effective and non-excessive amount of at least one cross-linking agent; and
homogenizing the crosslinked mixture to obtain the injectable composition.
2. The composition of claim 1 having a concentration of hyaluronic acid of between 10 and 40 mg/g.
3. The composition of claim 1 having a concentration of hyaluronic acid of between 20 and 30 mg/g.
4. The composition of claim 1 having a concentration of hyaluronic acid of 26 mg/g.
5. The composition of claim 1 , wherein the cross-linking agent is selected from epichlorohydrin, divinyl sulfone, 1,4-bis(2,3-epoxypropoxy) butane, 1,2-bis(2,3-epoxypropoxy)ethylene, 1-(2,3-epoxypropyl)-2,3-epoxycyclohexane, aldehydes, and mixtures thereof.
6. The composition of claim 1 , wherein the cross-linking agent is 1,4-butanediol diglycidyl ether (BDDE).
7. The composition of claim 1 wherein the first hyaluronic acid salt has a first intrinsic viscosity and the second hyaluronic acid salt has a second intrinsic viscosity different from the first intrinsic viscosity.
8. The composition of claim 1 wherein first hyaluronic acid salt and the second hyaluronic acid salt are mixed at a weight ratio of 90:10, respectively.
9. The composition of claim 1 , wherein the step of mixing the first hyaluronic acid salt and second hyaluronic acid salt is further conducted in an aqueous solution.
10. The composition of claim 9 , wherein the aqueous solution is a basic solution.
11. The composition of claim 1 , further comprising the step of, after homogenizing, sterilizing the injectable composition.
12. The composition of claim 11 , wherein the step of sterilizing comprises autoclaving the injectable composition.