IP Library › Granted Patent US 12,324,663
Granted Patent B2
US 12,324,663 · App. 15/310,253 · Granted Jun 10, 2025

Systems and methods for making and using sensors, probes, and other devices

Inventors: Qimin Quan (Somerville, MA); Wooyoung Hong (Gyeonggi-do, KR); Marko Loncar (Belmont, MA)
Assignee: President and Fellows of Harvard College
A61B5/1459A61B5/01A61B5/05A61B5/14546A61B5/14556A61B5/14735B82B1/001B82B3/0019G01H9/00G01N15/1429G01N15/1434G01N15/1484G01N21/553G01N21/6428G01N21/65G01N33/5308G01N33/54373A61B5/1455A61B2562/0238A61B2562/028A61B2562/0285A61B2562/029A61B2562/125G01N15/01G01N2015/1006G01N2201/08
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Quick Facts
Patent No.
US 12,324,663
App. No.
15/310,253
Granted
Jun 10, 2025
Kind
B2
Abstract

The present invention generally relates, in some aspects, to systems and methods for making and using sensors or other devices, such as optical components. One aspect is generally directed to a sensor or other device comprising a nanometer-sized portion. In some embodiments, the sensor can be used to determine various characteristics such as temperature, humidity, an electric field, a magnetic field, an analyte, or the like. For instance, in one embodiment, a portion of a sensor device may be inserted into a cell and used to study the cell, e.g., using optical techniques such as surface plasma resonance. In some embodiments, such sensors or other devices may comprise metal, glass, or other materials, which can be prepared using etching or other techniques.

Claims (28)

1. An article for inserting a particle into a cell, the article comprising:

a member configured to guide and/or direct light to analyze the cell, the member comprising a starting portion, an end portion configured for insertion into the cell while maintaining viability of the cell, and a tapered portion connecting the starting portion to the end portion, wherein (i) the starting portion has a substantially constant average cross-sectional diameter of at least about 100 micrometers and is configured for guiding the end portion into the cell, (ii) the end portion for insertion into the cell while maintaining viability of the cell has a substantially constant average cross-sectional diameter of less than 100 nm and a length of between about 750 micrometers and about 3 mm, and (iii) the tapered portion connecting the starting portion to the end portion has a length of no more than about 500 micrometers; and

at least one particle attached to the end portion of the member and configured to facilitate analysis of the cell by interacting with an analyte from the cell such that a characteristic of the analyte is determined by light propagated through the member, wherein (i) the at least one particle is positioned in an optically interrogatable position with respect to light transmitted through the member to the end portion, (ii) the at least one particle is attached to the end portion of the member via an adhesive positioned between the at least one particle and the end portion of the member, and (iii) the adhesive comprises at least one of a polymer or an epoxy,

wherein the member is formed by etching.

2. The article of claim 1 , wherein the member comprises a metal.

3. The article of claim 1 , wherein the member comprises glass.

4. The article of claim 1 , wherein the member is able to transmit light.

5. The article of claim 1 , wherein the at least one particle comprises a metal.

6. The article of claim 1 , wherein the at least one particle is at least partially coated with a reaction entity.

7. The article of claim 1 , wherein the end portion of the member is coated with a plurality of particles.

8. The article of claim 1 , wherein the end portion is at least partially inserted into the cell.

9. The article of claim 1 , wherein the at least one particle is in surface plasmon resonance (SPR) communication with the member.

10. The article of claim 5 , wherein the at least one particle comprises gold.

11. The article of claim 5 , wherein the at least one particle comprises silver.

12. The article of claim 5 , wherein the at least one particle comprises aluminum.

13. The article of claim 1 , wherein the at least one particle comprises a polymer, silica or glass.

14. The article of claim 1 , wherein the member comprises gold.

15. The article of claim 1 , wherein the member comprises silicon.

16. The article of claim 6 , wherein the reaction entity comprises a binding partner to which the analyte binds.

17. The article of claim 6 , wherein the reaction entity comprises a metal, a nucleic acid, an antibody, an aptamer, a sugar, a carbohydrate, a protein, a polymer, a catalyst, and/or a quantum dot.

18. The article of claim 6 , wherein the reaction entity comprises platinum.

19. The article of claim 6 , wherein the reaction entity comprises a hydrogel.

20. The article of claim 1 , wherein facilitating analysis of the cell comprises determining a vibration characteristic and/or a change in the vibration characteristic of the member.

21. The article of claim 20 , wherein the vibration characteristic and/or the change in the vibration characteristic of the member is due to a change in temperature, electric field, and/or magnetic field of the cell.

22. The article of claim 1 , wherein the adhesive comprises the polymer.

23. The article of claim 1 , wherein the adhesive is the epoxy.

24. The article of claim 1 , wherein the cell is a HeLa cell.

25. The article of claim 1 , wherein the member is configured to guide and/or direct light applied to the starting portion and directed to the cell via the tapered portion and the end portion.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 12, 2016
From: QUAN, QIMIN; HONG, WOOYOUNG; LONCAR, MARKO
To: PRESIDENT AND FELLOWS OF HARVARD COLLEGE
Reel/Frame 040707/0007 →
Continuity (2)
Provisional Application 61991842 · May 12, 2014
Related Publication 20170265788A1 · Sep 21, 2017
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