Method of making a hydrolyzed collagen gel
The method of making a hydrolyzed collagen gel is a manufacturing method for producing a hydrolyzed collagen gel to be used as a topical wound treatment. A first volume of purified water is initially heated to a temperature ranging from about 71° C. to about 77° C., and a hydrolyzed type I collagen powder is then mixed into the heated purified water to form a first mixture. An additive is mixed into the first mixture to form a second mixture, where the additive may be native collagen, at least one amino acid, least one therapeutic agent, gelatin, whey, hydrolyzed whey, polysulfated glycosaminoglycan, a glucosamine salt, glutamine, glycosaminoclycans, zinc, silver oxide alginates, cellulose, honey, mushroom extract and combinations thereof. A second volume of purified water is then added to the second mixture to form the hydrolyzed collagen gel product. Mixing is performed as a continuous, recirculating, temperature-monitored and temperature-maintained process.
1. A method of making a hydrolyzed collagen gel, comprising the steps of:
heating a first volume of water to a temperature ranging from about 71° C. to about 77° C.;
mixing a hydrolyzed type I collagen powder into the heated water to form a first mixture;
mixing an additive into the first mixture to form a second mixture, wherein the additive is selected from the group consisting of native collagen, at least one amino acid, least one therapeutic agent, gelatin, whey, hydrolyzed whey, polysulfated glycosaminoglycan, a glucosamine salt, glutamine, glycosaminoclycans, zinc, silver oxide alginates, cellulose, honey, mushroom extract and combinations thereof; and
adding a second volume of water to the second mixture to form a hydrolyzed collagen gel.
2. The method of making a hydrolyzed collagen gel as recited in claim 1 , wherein the step of heating the water comprises heating the water to a temperature of 74° C.
3. The method of making a hydrolyzed collagen gel as recited in claim 1 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in a propeller mixer with side sweeps.
4. The method of making a hydrolyzed collagen gel as recited in claim 3 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer at a propeller speed between 240 rpm and 1460 rpm.
5. The method of making a hydrolyzed collagen gel as recited in claim 4 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer with a side sweep frequency between 25 Hz and 35 Hz.
6. The method of making a hydrolyzed collagen gel as recited in claim 5 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer for a period of time ranging from about 20 minutes to about 30 minutes.
7. The method of making a hydrolyzed collagen gel as recited in claim 1 , wherein the hydrolyzed type I collagen powder has an average molecular weight ranging from about 1,000 Da to about 10,000 Da.
8. The method of making a hydrolyzed collagen gel as recited in claim 7 , wherein the hydrolyzed type I collagen powder has an average molecular weight ranging from about 2,000 Da to about 4,000 Da.
9. The method of making a hydrolyzed collagen gel as recited in claim 8 , wherein the hydrolyzed type I collagen powder has an average molecular weight ranging from about 2,500 Da to about 2,800 Da.
10. The method of making a hydrolyzed collagen gel as recited in claim 1 , wherein the step of mixing the additive into the first mixture comprises mixing the additive and the first mixture in the propeller mixer for a period of time ranging from about 8 minutes to about 12 minutes.
11. The method of making a hydrolyzed collagen gel as recited in claim 10 , wherein the step of mixing the additive into the first mixture comprises mixing the additive and the first mixture in the propeller mixer with a propeller speed of 800 rpm.
12. The method of making a hydrolyzed collagen gel as recited in claim 11 , wherein the step of mixing the additive into the first mixture comprises mixing the additive and the first mixture in the propeller mixer with a side sweep frequency ranging from about 25 Hz to about 35 Hz.
13. The method of making a hydrolyzed collagen gel as recited in claim 1 , wherein the step of adding the second volume of water to the second mixture to form the hydrolyzed collagen gel comprises adding the second volume of water to the second mixture to form the hydrolyzed collagen gel such that a concentration of the hydrolyzed type I collagen in the hydrolyzed collagen gel is about 67.00 wt %.
14. The method of making a hydrolyzed collagen gel as recited in claim 13 , wherein the step of adding the second volume of water to the second mixture to form the hydrolyzed collagen gel comprises adding the second volume of water to the second mixture to form the hydrolyzed collagen gel such that a concentration of the additive in the hydrolyzed collagen gel is 0.29 wt %.
15. A method of making a hydrolyzed collagen gel, comprising the steps of:
heating a first volume of water to a temperature ranging from about 71° C. to about 77° C.;
mixing a hydrolyzed type I collagen powder into the heated water to form a first mixture;
mixing an additive into the first mixture to form a second mixture, wherein the additive is selected from the group consisting of native collagen, at least one amino acid, least one therapeutic agent, gelatin, whey, hydrolyzed whey, polysulfated glycosaminoglycan, a glucosamine salt, glutamine, glycosaminoclycans, zinc, silver oxide alginates, cellulose, honey, mushroom extract and combinations thereof; and
adding a second volume of water to the second mixture to form a hydrolyzed collagen gel, wherein a concentration of the hydrolyzed type I collagen in the hydrolyzed collagen gel is about 67.00 wt %, a concentration of the additive in the hydrolyzed collagen gel is about 0.29 wt %, and a concentration of the water in the hydrolyzed collagen gel is about 32.71 wt %.
16. The method of making a hydrolyzed collagen gel as recited in claim 15 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in a propeller mixer with side sweeps.
17. The method of making a hydrolyzed collagen gel as recited in claim 16 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer at a propeller speed ranging from about 240 rpm to about 1460 rpm.
18. The method of making a hydrolyzed collagen gel as recited in claim 17 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer with a side sweep frequency ranging from about 25 Hz to about 35 Hz.
19. The method of making a hydrolyzed collagen gel as recited in claim 18 , wherein the step of mixing the hydrolyzed type I collagen powder into the heated water comprises mixing the hydrolyzed type I collagen powder and the heated water in the propeller mixer for a time period ranging from about 20 minutes to about 30 minutes, and the step of mixing the additive into the first mixture comprises mixing the additive and the first mixture in the propeller mixer with a propeller speed of about 800 rpm and with a side sweep frequency ranging from about 25 Hz to about 35 Hz.