Direct-fed microbials
The invention relates to direct-fed microbials for use in E. coli inhibition in animals. More particularly, the invention relates to isolated Bacillus strains 101, 235, 77, 177, and 102 and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. The invention can also be used for treatment of plants and in food processing.
1. A method of feeding an animal, the method comprising the step of administering to the animal a feed composition or drinking water comprising an effective amount of an additive comprising an isolated Bacillus strain selected from the group consisting of Bacillus strain 101 (NRRL No. B-67218), Bacillus strain 235 (NRRL No. B-67219), Bacillus strain 77 (NRRL No. B-67274), Bacillus strain 177 (NRRL No. B-67275), Bacillus strain 102 (NRRL No. B-67276), a strain having all of the identifying characteristics of Bacillus strain 101 (NRRL No. B-67218), a strain having all of the identifying characteristics of Bacillus strain 235 (NRRL No. B-67219), a strain having all of the identifying characteristics of Bacillus strain 77 (NRRL No. B-67274), a strain having all of the identifying characteristics of Bacillus strain 177 (NRRL No. B-67275), a strain having all of the identifying characteristics of Bacillus strain 102 (NRRL No. B-67276), and combinations thereof, wherein the Bacillus strain causes E. coli inhibition in the animal.
2. The method of claim 1 wherein the animal is selected from the group consisting of a poultry species, a porcine species, a bovine species, an ovine species, an equine species, and a companion animal.
3. The method of claim 2 wherein the poultry species is a broiler chicken.
4. The method of any one of claim 1 wherein the E. coli inhibition prevents E. coli disease in the animal.
5. The method of any one of claim 1 wherein the E. coli inhibition reduces E. coli disease in the animal.
6. The method of claim 2 wherein the animal is a porcine species and the porcine species is selected from the group consisting of a grow finish pig, a nursery pig, a sow, and a breeding stock pig.
7. The method of claim 1 wherein the Bacillus strain produces an enzyme selected from the group consisting of an a-galactosidase, a protease, a lipase, an amylase, a xylanase, a cellulase, and combinations thereof.
8. The method of claim 1 further comprising the step of administering to the animal another bacterial strain selected from the group consisting of another Bacillus strain, a lactic acid bacterial strain, and combinations thereof.
9. The method of claim 1 wherein the strain administered is Bacillus strain 101 (NRRL No. B-67218) or a strain having all of the identifying characteristics of Bacillus strain 101 (NRRL No. B-67218).
10. The method of claim 1 wherein the strain administered is Bacillus strain 235 (NRRL No. B-67219) or a strain having all of the identifying characteristics of Bacillus strain 235 (NRRL No. B-67219).
11. The method of claim 1 wherein Bacillus strain 101 (NRRL No. B-67218), or a strain having all of the identifying characteristics of Bacillus strain 101 (NRRL No. B-67218), and Bacillus strain 235 (NRRL No. B-67219), or a strain having all of the identifying characteristics of Bacillus strain 235 (NRRL No. B-67219), are administered in combination in a single composition.
12. The method of claim 1 wherein Bacillus strain 101 (NRRL No. B-67218), or a strain having all of the identifying characteristics of Bacillus strain 101 (NRRL No. B-67218), and Bacillus strain 235 (NRRL No. B-67219), or a strain having all of the identifying characteristics of Bacillus strain 235 (NRRL No. B-67219), are administered in combination in separate compositions.
13. The method of claim 1 wherein wherein the Bacillus strain is administered in the feed composition at a dose of about 1.0×10 3 CFU/gram of the feed composition to about 5.0×10 12 CFU/gram of the feed composition.
14. The method of claim 1 wherein the Bacillus strain is administered in the feed composition at a dose of about 1.0×10 3 CFU/gram of the feed composition to about 1.0 ×10 7 CFU/gram of the feed composition.
15. The method of claim 1 wherein the Bacillus strain is administered in the feed composition at a dose greater than about 7.0×10 4 CFU/gram of the feed composition.
16. The method of claim 1 wherein the Bacillus strain is administered in the feed composition at a dose of about 7.3×10 4 CFU/gram of the feed composition.
17. The method of claim 1 wherein the Bacillus strain is isolated from a high performing grow finish pig.
18. The method of claim 1 further comprising the step of administering an antibiotic to the animal.
19. The method of claim 1 further comprising the step of administering to the animal an enzyme selected from the group consisting of a galactosidase, a protease, a lipase, an amylase, a hemicellulase, an arabinoxylanase, a xylanase, a cellulase, an NSPase, a phytase, and combinations thereof.
20. The method of claim 19 wherein the enzyme is an NSPase or a phytase.