IP Library › Granted Patent US 12,369,604
Granted Patent B2
US 12,369,604 · App. 17/637,013 · Granted Jul 29, 2025

Methods of inhibition with microbial strains and antibiotics

Inventors: Michael R. King (Oak Creek, WI); Sona Son (Cudahy, WI); Kyle Leistikow (Cudahy, WI)
Assignee: MICROBIAL DISCOVERY GROUP, LLC
A23K10/18A23K20/189A61K35/742A61P31/04
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Quick Facts
Patent No.
US 12,369,604
App. No.
17/637,013
Granted
Jul 29, 2025
Kind
B2
Abstract

The invention relates to direct-fed microbials for use in E. coli and/or Clostridium inhibition in animals in combination with antibiotics. More particularly, the invention relates to isolated Bacillus strains 2, 3, 4, 5, 6, 7, 9, 57, 71, and 126, and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use.

Claims (26)

1. A method of enhancing the efficacy of an antibiotic treatment and inhibit the growth of antibiotic resistant bacteria in an animal, the method comprising the step of

administering to the animal a feed composition or drinking water comprising an effective amount of an additive, wherein said additive comprises an isolated Bacillus strain selected from the group consisting of Bacillus strain 2 deposited as NRRL No. B-67709 , Bacillus strain 3 deposited as NRRL No. B-67710 , Bacillus strain 4 deposited as NRRL No. B-67711 , Bacillus strain 5 deposited as NRRL No. B-67712 , Bacillus strain 6 deposited as NRRL No. B-67714 , Bacillus strain 7 deposited as NRRL No. B-67713 , Bacillus strain 9 deposited as NRRL No. B-67866 , Bacillus strain 57 deposited as NRRL No. B-67870 , Bacillus strain 71 deposited as NRRL No. B-67867 , Bacillus strain 126 deposited as NRRL No. B-67868, and combinations thereof, and

administering to the animal an antibiotic, wherein the administration of said additive in conjunction with said antibiotic enhances the efficacy of the antibiotic relative to administration of the antibiotic in the absence of the additive.

2. A method of enhancing the efficacy of an antibiotic treatment and inhibit the growth of antibiotic resistant bacteria in an animal, the method comprising the step of

administering to the animal a feed composition or drinking water comprising an effective amount of an additive, wherein said additive comprises an isolated Bacillus strain selected from the group consisting of Bacillus strain 2 deposited as NRRL No. B-67709, Bacillus strain 3 deposited as NRRL No. B-67710, Bacillus strain 4 deposited as NRRL No. B-67711, Bacillus strain 5 deposited as NRRL No. B-67712, Bacillus strain 6 deposited as NRRL No. B-67714, and Bacillus strain 7 deposited as NRRL No. B-67713, and combinations thereof, and

administering to the animal an antibiotic, wherein the administration of said additive in conjunction with said antibiotic enhances the efficacy of the antibiotic relative to administration of the antibiotic in the absence of the additive.

3. The method of claim 1 further comprising the step of administering to the animal another different bacterial strain selected from the group consisting of another Bacillus strain, a lactic acid bacterial strain, and combinations thereof.

4. The method of claim 2 wherein the strain administered is Bacillus strain 2 deposited as NRRL No. B-67709.

5. The method of claim 2 wherein the strain administered is Bacillus strain 3 deposited as NRRL No. B-67710.

6. The method of claim 2 wherein the strain administered is Bacillus strain 4 deposited as NRRL No. B-67711.

7. The method of claim 2 wherein the strain administered is Bacillus strain 5 deposited as NRRL No. B-67712.

8. The method of claim 2 wherein the strain administered is Bacillus strain 6 deposited as NRRL No. B-67714.

9. The method of claim 2 wherein the strain administered is Bacillus strain 7 deposited as NRRL No. B-67713.

10. The method of claim 1 wherein the Bacillus strain is administered in the feed composition at a dose of about 1.0×10 3 CFU/gram of the feed composition to about 5.0×10 12 CFU/gram of the feed composition.

11. The method of claim 1 further comprising the step of administering to the animal an enzyme selected from the group consisting of a galactosidase, a protease, a lipase, an amylase, a hemicellulase, an arabinoxylanase, a xylanase, a cellulase, an NSPase, a phytase, and combinations thereof.

12. The method of claim 1 wherein the animal is a sow and the Bacillus strain is administered during lactation.

13. The method of claim 1 wherein the animal is a sow and the Bacillus strain is administered during gestation.

14. The method of claim 1 wherein the feed composition is administered daily to the animal.

15. The method of claim 1 wherein the animal is selected from the group consisting of a chicken, a pig, a horse, a pony, a cow, a turkey, a goat, a sheep, a quail, a pheasant, an ostrich, a duck, a fish, a crustacean, and combinations thereof.

16. The method of claim 1 further comprising the step of administering an antibiotic wherein the antibiotic is selected from the group consisting of tylosin, tilmicosin, avilamycin, gentamycin, carbadox, tiamulin, and neomycin.

17. The method of claim 1 wherein the strain administered is Bacillus strain 9 deposited as NRRL No. B-67866.

18. The method of claim 1 wherein the strain administered is Bacillus strain 57 deposited as NRRL No. B-67870.

19. The method of claim 1 wherein the strain administered is Bacillus strain 71 deposited as NRRL No. B-67867.

20. The method of claim 1 wherein the strain administered is Bacillus strain 126 deposited as NRRL No. B-67868.

21. A commercial package comprising an isolated Bacillus strain selected from the group consisting of Bacillus strain 2 deposited as NRRL No. B-67709 , Bacillus strain 3 deposited as NRRL No. B-67710 , Bacillus strain 4 deposited as NRRL No. B-67711 , Bacillus strain 5 deposited as NRRL No. B-67712 , Bacillus strain 6 deposited as NRRL No. B-67714 , Bacillus strain 7 deposited as NRRL No. B-67713 , Bacillus strain 9 deposited as NRRL No. B-67866 , Bacillus strain 57 deposited as NRRL No. B-67870, Bacillus strain 71 deposited as NRRL No. B-67867 , Bacillus strain 126 deposited as NRRL No. B-67868, and combinations thereof.

22. The method of claim 1 wherein the animal is selected from the group consisting of a poultry species, a porcine species, a caprine species, a bovine species, an ovine species, an equine species, and a companion animal.

Continuity (2)
Provisional Application 62890267 · Aug 22, 2019
Related Publication 20220295828A1 · Sep 22, 2022
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