Microbials for feed
The invention relates to direct-fed microbials for use in E. coli, Salmonella , and/or Campylobacter inhibition in animals. More particularly, the invention relates to isolated Bacillus strains A12, 54, 681, 101, 235, and 721, and strains having all of the identifying characteristics of these strains, for a use comprising the above-mentioned use. The invention also relates to the use of isolated Bacillus strains 681,721, A12, 54, 86, 300, 101, 102, 235, and 177, and strains having all of the identifying characteristics of these strains, to prevent or reduce prolapse in an animal.
1. A method of feeding an animal, the method comprising the step of administering to the animal a feed composition or drinking water comprising an effective amount of an additive comprising an isolated Bacillus subtilis strain selected from the group consisting of strains A12 (NRRL No. B-67516), 54 (NRRL No. B-67517), 681 (NRRL No. B-67515), 721 (NRRL No. B-67514), and combinations thereof, wherein the Bacillus subtilis strain reduces prolapse in the animal, optionally wherein said additive further comprises one or more Bacillus strains selected from Bacillus subtilis strain 300 (NRRL No. B-50943), Bacillus subtilis strain 101 (NRRL No. B-67218), Bacillus subtilis strain 235 (NRRL No. B-67219), Bacillus subtilis strain 86 (NRRL No. B-50944), Bacillus pumilus strain 102 (NRRL No. B-67276), and Bacillus pumilus strain 177 (NRRL No. B-67275).
2. The method of claim 1 wherein the prolapse is selected from the group consisting of vaginal prolapse, uterine prolapse, rectal prolapse, and anal prolapse.
3. The method of claim 2 wherein the prolapse is selected from the group consisting of vaginal prolapse and uterine prolapse.
4. The method of claim 1 wherein the animal is selected from the group consisting of a poultry species, a porcine species, a caprine species, a bovine species, an ovine species, an equine species, and a companion animal.
5. The method of claim 4 wherein the animal is a porcine species and the porcine species is selected from the group consisting of a grow finish pig, a nursery pig, a sow, and a breeding stock pig.
6. The method of claim 1 wherein the Bacillus subtilis strain produces an enzyme selected from the group consisting of an α-galactosidase, a protease, a lipase, an amylase, a xylanase, a cellulase, and combinations thereof.
7. The method of claim 1 further comprising the step of administering to the animal another bacterial strain selected from the group consisting of another Bacillus strain, a lactic acid bacterial strain, and combinations thereof.
8. The method of claim 1 wherein the Bacillus subtilis strain is administered in the feed composition or drinking water at a dose of about 1.0×10 3 CFU/gram of the feed composition to about 5.0×10 12 CFU/gram of the feed composition.
9. The method of claim 1 wherein the Bacillus subtilis strain is administered in the feed composition or drinking water at a dose of about 1.0×10 3 CFU/gram of the feed composition to about 1.0×10 7 CFU/gram of the feed composition.
10. The method of claim 1 further comprising the step of administering an antibiotic to the animal.
11. The method of claim 1 further comprising the step of administering to the animal an enzyme selected from the group consisting of a galactosidase, a protease, a lipase, an amylase, a hemicellulase, an arabinoxylanase, a xylanase, a cellulase, an NSPase, a phytase, and combinations thereof.
12. The method of claim 11 wherein the enzyme is an NSPase or a phytase.
13. The method of claim 1 wherein the animal is selected from the group consisting of a chicken, a pig, a horse, a pony, a cow, a turkey, a goat, a sheep, a quail, a pheasant, an ostrich, a duck, a fish, a crustacean, and combinations thereof.
14. The method of claim 1 wherein the additive comprises the Bacillus subtilis strain A12 (NRRL No. B-67516).
15. The method of claim 1 wherein the additive comprises the Bacillus subtilis strain 54 (NRRL No. B-67517).
16. The method of claim 1 wherein the additive comprises the Bacillus subtilis strain 681 (NRRL No. B-67515).
17. The method of claim 1 wherein the additive comprises the Bacillus subtilis strain 721 (NRRL No. B-67514).