Vaccination for
View Patent ↗The present invention include a method of protecting an animal against Lawsonia intracellularis infections, the method including administering a dose of live virulent L. intracellularis to the animal, allowing a subclinical L. intracellularis infection to develop in the animal, and administering one or more antibiotics to the animal, wherein the one or more antibiotic is administered in a dose sufficient to abbreviate the subclinical L. intracellularis infection.
1. A method of producing an immune response to Lawsonia intracellularis in a pig, the method comprising:
administering a subclinical dose of live virulent Lawsonia intracellularis to the pig;
wherein the dose of live virulent Lawsonia intracellularis is 10 4 to 10 6 Lawsonia intracellularis organisms per pig;
allowing a subclinical Lawsonia intracellularis infection to develop in the pig;
wherein a subclinical infection is an infection in which diarrhea, gauntness, and depression are absent; and
administering one or more antibiotic to the pig, wherein the one or more antibiotic is administered in a dose sufficient to abbreviate the subclinical Lawsonia intracellularis infection.
2. The method of claim 1 , wherein the pig develops an immune response comprising serum IgG antibodies to Lawsonia intracellularis.
3. The method of claim 1 , wherein the pig develops an immune response comprising cell-mediated immunity to Lawsonia intracellularis.
4. The method of claim 1 , wherein the one or more antibiotic comprises carbadox.
5. The method of claim 1 , wherein the one or more antibiotic is administered to the pig in the feed.
6. The method of claim 1 , wherein the one or more antibiotic is administered to the pig in the drinking water.
7. The method of claim 1 , wherein the live virulent Lawsonia intracellularis is delivered orally to the pig in the drinking water or per os.
8. The method of claim 1 , wherein the one or more antibiotic is administered to the pig beginning at 10 or more days after the administration of the dose of live virulent Lawsonia intracellularis or beginning at 14 or more days after the administration of the dose of live virulent Lawsonia intracellularis.
9. The method of claim 1 , wherein the one or more antibiotic is administered to the pig beginning more than 7 days after the administration of the dose of live virulent Lawsonia intracellularis.
10. The method of claim 1 , wherein the dose of live virulent Lawsonia intracellularis is obtained from an autogenous source.
11. The method of claim 1 wherein the subclinical dose of live virulent Lawsonia intracellularis comprises an intestinal homogenate.
12. The method of claim 1 , wherein a time interval of more than 7 days but no more that 21 days is allowed to pass between administration of the dose of live virulent Lawsonia intracellularis and initiation of administration of the one or more antibiotic.
13. The method of claim 1 , wherein the immune response reduces the symptoms of future Lawsonia intracellularis infections in the pig.
14. A method of producing an immune response to Lawsonia intracellularis in a pig, the method comprising:
administering a subclinical dose of live virulent Lawsonia intracellularis to the pig;
wherein the dose of live virulent Lawsonia intracellularis is 10 4 to 10 6 Lawsonia intracellularis organisms per pig;
allowing a subclinical Lawsonia intracellularis infection to develop in the pig;
wherein a subclinical infection is an infection in which diarrhea, gauntness, and depression are absent; and
administering one or more antibiotic to the pig, wherein the one or more antibiotic is administered in a dose sufficient to abbreviate the subclinical Lawsonia intracellularis infection;
wherein the one or more antibiotic is administered to the pig beginning more than 7 days after the administration of the dose of live virulent Lawsonia intracellularis.
15. The method of claim 14 , wherein the pig develops an immune response comprising serum IgG antibodies to Lawsonia intracellularis and/or cell-mediated immunity to Lawsonia intracellularis.
16. The method of claim 14 , wherein the one or more antibiotic comprises carbadox.
17. The method of claim 14 , wherein the one or more antibiotic is administered to the pig beginning at 10 or more days after the administration of the dose of live virulent Lawsonia intracellularis or beginning at 14 or more days after the administration of the dose of live virulent Lawsonia intracellularis.
18. The method of claim 14 , wherein a time interval of more than 7 days but no more that 21 days is allowed to pass between administration of the dose of live virulent Lawsonia intracellularis and initiation of administration of the one or more antibiotic.
19. The method of claim 14 wherein the immune response reduces the symptoms of future Lawsonia intracellularis infections in the pig.
20. A method of producing an immune response to Lawsonia intracellularis in a pig, the method comprising:
administering a subclinical dose of live virulent Lawsonia intracellularis to the pig;
wherein the dose of live virulent Lawsonia intracellularis is 10 4 to 10 6 Lawsonia intracellularis organisms per pig;
allowing a subclinical Lawsonia intracellularis infection to develop in the pig;
wherein a subclinical infection is an infection in which diarrhea, gauntness, and depression are absent; and
administering one or more antibiotic to the pig, wherein the one or more antibiotic is administered in a dose sufficient to abbreviate the subclinical Lawsonia intracellularis infection;
wherein a time interval of more than 7 days but no more that 21 days is allowed to pass between administration of the dose of live virulent Lawsonia intracellularis and initiation of administration of the one or more antibiotic.
21. The method of claim 20 , wherein the one or more antibiotic comprises carbadox.
22. The method of claim 20 , wherein the dose of live virulent Lawsonia intracellularis is obtained from an autogenous source.