Method and composition for reducing
Bacillus strains that inhibit pathogenic swine E. coli and/or improve performance are provided. Inhibition of pathogenic swine E. coli decreases E. coli disease. At least one strain enhanced swine performance by improving average daily gain, feed efficiency, and feed intake. Preferred Bacillus strains are of species that are included on the GRAS list. Bacillus species are sporeformers and therefore are highly stable and can be fed to swine.
1. An isolated microorganism of the genus Bacillus selected from the group consisting of strains 3A-P4 ATCC PTA-6506, 15A-P4 ATCC PTA-6507, and 22C-P1 ATCC PTA-6508.
2. The microorganism of claim 1 , wherein the microorganism is capable of at least one of the following:
(A) inhibiting E. coli disease in animals fed the microorganism when compared to animals not fed the microorganism, and
(B) improving performance in animals fed the microorganism compared to animals not fed the microorganism, wherein the improvement in performance includes an improvement in at least one of average daily gain (ADG), weight, mortality, feed conversion, and feed intake.
3. The microorganism of claim 1 , wherein the microorganism is strain 15A-P4 ATCC PTA-6507.
4. A combination of microorganisms comprising at least two microorganisms of claim 1 .
5. The combination of claim 4 , wherein the combination comprises strains 22C-P1 ATCC PTA-6508 and 15A-P4 ATCC PTA-6507.
6. The combination of claim 5 , wherein the combination comprises 90% of the total count of strain 22C-P1 ATCC PTA-6508 and 10% of the total count of strain 15A-P4 ATCC PTA-6507.
7. The combination of claim 6 , further comprising a feed, wherein the combination has a count of 5.1×10 8 CFU/g of feed.
8. The combination of claim 5 , wherein combination comprises 10% of the total count of strain 22C-P1 ATCC PTA-6508 and 90% of the total count of strain 15A-P4 ATCC PTA-6507.
9. The combination of claim 8 , further comprising a feed, wherein the combination has a count of 1×10 6 CFU/g of feed.
10. An isolated Bacillus strain 3A-P4 ATCC PTA-6506.
11. An isolated Bacillus strain 15A-P4 ATCC PTA-6507.
12. An isolated Bacillus strain 22C-P1 ATCC PTA-6508.
13. A combination of microorganisms of the genus Bacillus , the combination comprising strains 22C-P1 ATCC PTA-6508 and 15A-P4 ATCC PTA-6507.
14. A method of feeding swine, the method comprising feeding to the swine the microorganism of claim 1 .
15. The method of claim 14 , wherein the microorganism is strain 15A-P4 ATCC PTA-6507.
16. The method of claim 14 , wherein the microorganism comprises at least two strains.
17. The method of claim 16 , wherein the strains comprise strain 22C-P1 ATCC PTA-6508 and strain 15A-P4 ATCC PTA-6507.
18. The method of claim 17 , wherein the microorganism comprise 90% of the total count of strain 22C-P1 ATCC PTA-6508 and 10% of the total count of strain 15A-P4 ATCC PTA-6507.
19. The method of claim 17 , wherein the microorganism comprise 10% of the total count of strain 22C-P1 ATCC PTA-6508 and 90% of the total count of strain 15A-P4 ATCC PTA-6507.
20. A method of forming a direct-fed microbial, the method comprising:
(a) growing, in a liquid nutrient broth, the microorganism of claim 1 ; and
(b) separating the microorganism from the liquid to form the direct-fed microbial.
21. The method of claim 20 , wherein the microorganism is strain 15A-P4 ATCC PTA-6507.
22. The method of claim 21 , further comprising:
growing, in a liquid broth, strain 22C-P1 ATCC PTA-6508; and
combining strains 15A-P4 ATCC PTA-6507 and 22C-P1 ATCC PTA-6508.
23. A method of feeding swine, the method comprising feeding to the swine the combination of claim 13 .