Hybrid material for chromatographic separations comprising a superficially porous core and a surrounding material
The present invention provides novel chromatographic materials, e.g., for chromatographic separations, processes for their preparation and separations devices containing the chromatographic materials. The preparation of the inorganic/organic hybrid materials of the invention wherein a surrounding material is condensed on a superficially porous hybrid core material will allow for families of different hybrid packing materials to be prepared from a single core hybrid material. Differences in hydrophobicity, ion-exchange capacity, chemical stability, surface charge or silanol activity of the surrounding material may be used for unique chromatographic separations of small molecules, carbohydrates, antibodies, whole proteins, peptides, and/or DNA.
1. An inorganic/organic hybrid material comprising an inorganic/organic hybrid surrounding material and a superficially porous core, wherein the inorganic/organic surrounding material is derived from (a) condensation of one or more organofunctional silanes or condensation of one or more organofunctional silanes and tetraalkoxysilane on the surface of the superficially porous core, or (b) application of a partially condensed organofunctional silane, a mixture of two or more organofunctional silanes, or a mixture of one or more organofunctional silanes with a tetraalkoxysilane on the surface of the superficially porous core wherein said inorganic/organic hybrid material has been surface modified with a surface modifier selected from the group consisting of octyltrichlorosilane, octadecyltrichlorosilane, octyldimethylchlorosilane and octadecyldimethylchlorosilane.