Synthetic surfaces for culturing stem cell derived oligodendrocyte progenitor cells
Synthetic surfaces suitable for culturing stem cell derived oligodendrocyte progenitor cells contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived oligodendrocyte progenitor cells in chemically defined media.
1. A method for culturing human pluripotent stem cell-derived oligodendrocyte progenitor cells, comprising depositing a suspension comprising the human pluripotent stem cell-derived oligodendrocyte progenitor cells on a polymer material, and culturing the deposited human pluripotent stem cell-derived oligodendrocyte progenitor cells in a cell culture medium, wherein the polymer material comprises a diacrylate or a dimethacrylate.
2. The method of claim 1 , wherein the dimethacrylate is glycerol dimethacrylate.
3. The method of claim 1 , wherein the diacrylate is 1,6-hexanediol diacrylate.
4. The method of claim 1 , wherein the diacrylate is tetra(ethylene glycol) diacrylate.
5. The method of claim 1 , wherein the polymer material comprises about 70% glycerol dimethacrylate.
6. The method of claim 1 , wherein the human pluripotent stem cells are human sells induced pluripotent stem (iPS) cells.
7. The method of claim 1 , wherein the human pluripotent stem cells are human embryonic stem (hES) cells.
8. The method of claim 1 , wherein the cell culture medium is a chemically defined medium.