Methods for making definitive endoderm using at least GDF-8
The present invention is directed to methods to differentiate pluripotent stem cells. In particular, the present invention is directed to methods and compositions to differentiate pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage comprising culturing the pluripotent stem cells in medium comprising a sufficient amount of GDF-8 to cause the differentiation of the pluripotent stem cells into cells expressing markers characteristic of the definitive endoderm lineage.
1. A method to differentiate pluripotent stem cells into definitive endoderm cells comprising culturing the pluripotent stem cells with a medium lacking activin A, and supplemented with GDF-8 and 14-Methyl-3,5,7,14,18,24,28-heptaazatetracyclo[20.3.1.1˜2,6˜.1˜8,12˜]octacosa-1(26),2(28),3,5,8(27),9,11,22,24-nonaen-17-one.
2. The method of claim 1 , wherein the pluripotent stem cells are human pluripotent stem cells.
3. The method of claim 2 , wherein the human pluripotent stem cells are human embryonic stem cells.
4. The method of claim 1 , wherein the medium is further supplemented with EGF, FGF, PDGF-A, VEGF, PDGF-D and muscimol.
5. The method of claim 1 , wherein the medium is serum-free.
6. The method of claim 1 , wherein the medium is further supplemented with FGF.
7. The method of claim 1 , wherein the medium comprises from about 5 ng/ml to about 500 ng/ml of GDF-8.
8. The method of claim 1 , wherein the cells are cultured for about one day to about three days.
9. The method of claim 1 , wherein the medium further contains one or more compound selected from the group consisting of: EGF, FGF4, PDGF-A, PDGF-B, PDGF-C, PDGF-D, VEGF, muscimol, PD98059, LY294002, U0124, U0126, and sodium butyrate.
10. The method of claim 9 , wherein the EGF is used at a concentration from about 5 ng/ml to about 500 ng/ml.
11. The method of claim 9 , wherein the FGF4 is used at a concentration from about 5 ng/ml to about 500 ng/ml.
12. The method of claim 9 , wherein one or more of PDGF-A, PDGF-B, PDGF-C and PDFG-D is used at a concentration from about 5 ng/ml to about 500 ng/ml.
13. The method of claim 9 , wherein the VEGF is used at a concentration from about 5 ng/ml to about 500 ng/ml.
14. The method of claim 9 , wherein the muscimol is used at a concentration from about 1 μM to about 200 μM.
15. The method of claim 9 , wherein the PD98059 is used at a concentration from about 0.1 μM to about 10 μM.
16. The method of claim 9 , wherein the LY294002 is used at a concentration from about 0.25 μM to about 25 μM.
17. The method of claim 9 , wherein one or more of the U0124 and U0126 is used at a concentration from about 0.1 μM to about 10 μM.
18. The method of claim 9 , wherein the sodium butyrate is used at a concentration from about 0.05 μM to about 5 μM.
19. The method of claim 1 , wherein the medium lacks Wnt3a.