Antifreeze proteins for use in downhole fluids
Antifreeze proteins having an ice-binding site, such as those derived from the Marinomonas primoryensis bacterium, may be used in additive compositions, fluid compositions, and methods for depressing the freezing point of the fluid compositions. The fluid composition may include the antifreeze protein and a base fluid, such as drilling fluids, servicing fluids, production fluids, completion fluids, injection fluids, refinery fluids, and combinations thereof.
1. An additive composition for a base fluid selected from the group consisting of a drilling fluid, a completion fluid, a production fluid, a servicing fluid, an injection fluid, a refinery fluid, and combinations thereof; wherein the additive comprises an antifreeze protein that is at least 75% homologous to the amino acid sequence of SEQ ID NO:2, and wherein the antifreeze protein comprises a polyhistadine tag attached to a C-terminal end of the antifreeze protein.
2. The additive composition of claim 1 , wherein the amount of the antifreeze protein within the additive composition ranges from about 1 ppm to about 10 ppt as compared to the total base fluid.
3. The additive composition of claim 1 , further comprising an additive selected from the group consisting of a winterizing agent, a kinetic hydrate Inhibitor, a pour point depressant, an anti-agglomerant, a fracturing fluid additive, and combinations thereof.
4. The additive composition of claim 3 , wherein the winterizing agent is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, ethylene glycol, propylene glycol, butylene glycol, hexylene glycol, diethylene glycol, dipropylene glycol, triethylene glycol, tripropylene glycol, propylene glycol methyl ether, dipropylene glycol methyl ether, tripropylene glycol methyl ether, propylene glycol propyl ether, dipropylene glycol propyl ether, tripropylene glycol propyl ether, propylene glycol butyl ether, dipropylene glycol butyl ether, tripropylene glycol butyl ether, propylene glycol phenyl ether, dipropylene glycol dimethyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol butyl ether, diethylene glycol hexyl ether, ethylene glycol propyl ether, ethylene glycol butyl ether, ethylene glycol hexyl ether, triethylene glycol methyl ether, triethylene glycol ethyl ether, triethylene glycol butyl ether, ethylene glycol phenyl ether, ethylene glycol ethyl ether, polyethylene glycol, monosaccharide, polypropylene glycol, dimethyl sulfoxide, glycerol, glycerine, copolymers thereof, and combinations thereof.
5. A fluid composition comprises:
a base fluid selected from the group consisting of drilling fluids, servicing fluids, production fluids, completion fluids, injection fluids, refinery fluids, and combinations thereof; and
an antifreeze protein that is at least 75% homologous to the amino acid sequence of SEQ ID NO:2, and wherein the antifreeze protein comprises a polyhistadine tag attached to a C-terminal end of the antifreeze protein.
6. The fluid composition of claim 5 , wherein the amount of the antifreeze protein within the additive composition ranges from about 1 ppm to about 10 ppt as compared to the total base fluid.
7. The fluid composition of claim 5 , wherein the fluid composition further comprises an additive selected from the group consisting of a winterizing agent, a kinetic hydrate Inhibitor, a pour point depressant, an anti-agglomerant, a fracturing fluid additive, and combinations thereof.
8. The fluid composition of claim 7 , wherein the winterizing agent is selected from the group consisting of methanol, ethanol, propanol, isopropanol, butanol, ethylene glycol, propylene glycol, butylene glycol, hexylene glycol, diethylene glycol, dipropylene glycol, triethylene glycol, tripropylene glycol, propylene glycol methyl ether, dipropylene glycol methyl ether, tripropylene glycol methyl ether, propylene glycol propyl ether, dipropylene glycol propyl ether, tripropylene glycol propyl ether, propylene glycol butyl ether, dipropylene glycol butyl ether, tripropylene glycol butyl ether, propylene glycol phenyl ether, dipropylene glycol dimethyl ether, diethylene glycol ethyl ether, diethylene glycol methyl ether, diethylene glycol butyl ether, diethylene glycol hexyl ether, ethylene glycol propyl ether, ethylene glycol butyl ether, ethylene glycol hexyl ether, triethylene glycol methyl ether, triethylene glycol ethyl ether, triethylene glycol butyl ether, ethylene glycol phenyl ether, ethylene glycol ethyl ether, polyethylene glycol, monosaccharide, polypropylene glycol, dimethyl sulfoxide, glycerol, glycerine, copolymers thereof, and combinations thereof.
9. The fluid composition of claim 5 , wherein the fluid composition further comprises a substance selected from the group consisting of hydrates, scale, and combinations thereof; and wherein the fluid composition comprises the substance in a reduced amount as compared to an identical fluid composition absent the antifreeze protein.