Method of identifying a biologically-active composition from a biofilm
The subject invention provides materials and methods that effectively support innate immunity and/or disperse pathogenic biofilms using readily available, nontoxic, natural substances, while supporting restoration of normal microbiotic homeostasis. In one embodiment, the subject invention provides anti-biofilm compositions comprising one or more probiotic organisms, anti-microbial honey, and other ingredients such as prebiotic compounds, other hive products, green tea derivatives, other plant derivatives, and vitamin D3.
1. A composition for oral consumption comprising a biologically-active composition, wherein the biologically-active composition is obtained by a method comprising the steps of:
(a) growing biofilm cellular mass, which has biological activity, in a biological growth medium;
(b) rendering said biofilm cellular mass non-viable;
and
(c) combining said non-viable biofilm cellular mass with an edible material to form said edible composition that comprises said non-viable biofilm cellular mass, wherein the microbe is a Lactobacillus.
2. The composition, according to claim 1 , wherein the biological activity of said biofilm cellular mass is anti-microbial activity or anti-inflammatory activity.
3. A composition for oral consumption comprising non-viable biologically-active biofilm cellular mass and further comprising an edible material, wherein the microbe is a Lactobacillus.
4. The composition, according to claim 3 , which is a probiotic composition.
5. The composition, according to claim 3 , wherein the composition is formulated for human consumption.
6. The composition, according to claim 3 , wherein the biological activity of said biofilm cellular mass is anti-microbial activity and/or anti-inflammatory activity.
7. The composition, according to claim 3 , comprising a biosurfactant produced by the biofilm.
8. The composition, according to claim 3 , wherein the edible material comprises ingredients that are generally regarded as safe (GRAS).
9. The composition, according to claim 3 , comprising one or more components selected from prebiotics, hive products, plant derivatives and vitamin D3.