Process for producing a PUFA-containing feedstuff by extruding a PUFA-containing biomass
In accordance with the invention, it was found, surprisingly, that a polyunsaturated fatty acids (PUFAs)-comprising biomass with other feedstuff components can be extruded at a low energy input of 12-28 Wh/kg to give an extrudate with a very high oil load capacity.
1. A feedstuff extrudate comprising a PUFA-comprising biomass, wherein the PUFA-comprising biomass comprises microbial cells of the taxon Labyrinthulomycetes, and has a sulphate content of 25 to 60 g/kg based on dry matter, and wherein the feedstuff extrudate has an oil load capacity of at least 0.35 g of oil per g of feedstuff extrudate.
2. The feedstuff extrudate of claim 1 , wherein said biomass comprises a sulphate content 25 to 50 g/kg based on the dry matter.
3. The feedstuff extrudate of claim 1 , wherein the PUFA-comprising biomass comprises microbial cells of the family Thraustochytriaceae.
4. The feedstuff extrudate of claim 1 , wherein the PUFA-comprising biomass comprises microbial cells of the genera: Thraustochytrium, Schizochytrium, Aurantiochytrium, Oblongichytrium or Ulkenia.
5. The feedstuff extrudate of claim 1 , wherein the PUFA-comprising biomass comprises microbial cells of the species Aurantiochytrium limacinum.
6. The feedstuff extrudate of claim 1 , wherein the biomass has a sulphate content of 25-35 g/kg based on the dry matter.
7. The feedstuff extrudate of claim 1 , comprising:
a) a total protein content of 30 to 60% by weight;
b) a total fat content of 15 to 35% by weight;
c) a total starch content of not more than 25% by weight; and
d) a biomass content of 2 to 22% by weight.
8. The feedstuff extrudate of claim 7 , further comprising one or more of:
e) a polyunsaturated fatty acid (PUFAs) content of 2 to 12% by weight;
f) an omega-3 fatty acid content of 1 to 6% by weight; and
g) a DHA content of 0.5 to 3% by weight.
9. The feedstuff extrudate of claim 1 , comprising:
a) a total protein content of 40 to 50% by weight;
b) a total fat content of 22 to 28% by weight;
c) a total starch content of 7 to 13% by weight;
d) a Thraustochytriaceae biomass content of 10 to 16% by weight;
and further comprising one or more of the following:
e) a polyunsaturated fatty acid (PUFA) content of 5 to 8% by weight;
f) an omega-3 fatty acid content of 2.5 to 4% by weight; and
g) a DHA content of 1.2 to 2.0% by weight.
10. The feedstuff extrudate of claim 4 , wherein the PUFA-comprising biomass has a sulphate content of 25 to 40 g/kg based on the dry matter.
11. The feedstuff extrudate of claim 10 , further comprising:
a) a total protein content of 35 to 55% by weight;
b) a total fat content 18 to 32% by weight; and
c) a total starch content of 5 to 15% by weight.
12. The feedstuff extrudate of claim 11 , further comprising:
d) a Thraustochytriaceae biomass content of 4 to 20% by weight;
e) a polyunsaturated fatty acid (PUFAs) content of 3 to 10% by weight;
f) an omega-3 fatty acid content of 2 to 4.5% by weight; and
g) a DHA content of 0.8 to 2.5% by weight.
13. A method of growing animals by feeding them a feedstuff extrudate comprising a PUFA-comprising biomass, wherein the PUFA-comprising biomass comprises microbial cells of the taxon Labyrinthulomycetes and has a sulphate content of 25 to 60 g/kg based on dry matter, and wherein the feedstuff extrudate has an oil load capacity of at least 0.35 g of oil per g of feedstuff extrudate.
14. The method of growing animals of claim 13 , wherein the PUFA-comprising biomass comprises microbial cells of the family Thraustochytriaceae and has a sulphate content of 25 to 50 g/kg, based on the dry matter and wherein the feedstuff extrudate comprises:
a) a total protein content of 30 to 60% by weight;
b) a total fat content of 15 to 35% by weight;
c) a total starch content of not more than 25% by weight; and
d) a biomass content of 2 to 22% by weight.
15. The method of growing animals of claim 13 , wherein the biomass comprises microbial cells of the species Aurantiochytrium limacinum and wherein the feedstuff extrudate further comprises one or more of the following:
e) a polyunsaturated fatty acid (PUFAs) content of 2 to 12% by weight;
f) an omega-3 fatty acid content of 1 to 6% by weight; and
g) a DHA content of 0.5 to 3% by weight.
16. The method of claim 14 , wherein the PUFA-comprising biomass has a sulphate content of 25 to 40 g/kg based on the dry matter.
17. The method of claim 16 , wherein the feedstuff extrudate further comprises:
a) a total protein content of 35 to 55% by weight;
b) a total fat content 18 to 32% by weight; and
c) a total starch content of 5 to 15% by weight.
18. The method of claim 17 , wherein the feedstuff extrudate further comprises:
d) a Thraustochytriaceae biomass content, of 4 to 20% by weight;
e) a polyunsaturated fatty acid (PUFAs) content of 3 to 10% by weight;
f) an omega-3 fatty acid content of 2 to 4.5% by weight; and
g) a DHA content of 0.8 to 2.5% by weight.