Stabilising fatty acid compositions
Assay method, comprising providing a plant capable of expressing a delta-12 desaturase, wherein said delta-12 desaturase has at least 50% total amino acid sequence identity to at least one of the sequences SEQ ID NO. 328 to 336, and/or at least 59% total amino acid sequence similarity to at least one of the sequences SEQ ID NO. 328 to 336, and wherein the plant is also capable of expressing at least one or more enzymes of unsaturated fatty acid metabolism, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is supposedly connected to a plant metabolic property, growing the plant, and measuring said plant metabolic property for said plant.
1. An assay method for simulating a plant metabolic property of a plant grown under field conditions, comprising:
i) providing a transgenic plant of order Brassicales expressing a delta-12 desaturase heterologous to the transgenic plant, wherein said delta-12 desaturase has at least 96% total amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 328,
and wherein the transgenic plant also expresses at least one or more enzymes of unsaturated fatty acid metabolism heterologous to the transgenic plant, of which enzymes at least one is capable of using linoleic acid as a substrate, and of which enzymes at least one is connected to a plant metabolic property,
il) growing the transgenic plant under greenhouse conditions;
iii) measuring said plant metabolic property for said transgenic plant, wherein the measured metabolic property simulates the plant metabolic property measurement for the transgenic plant grown under field conditions; and
iv) selecting transgenic offspring of the transgenic plant for field trials and conducing field trials on said selected transgenic offspring to produce a commercially viable transgenic plant variety of order Brassicales having said simulated plant metabolic property, wherein number of field trials required for development of said commercially viable transgenic plant variety is reduced compared to a corresponding control plant not expressing said delta-12 desaturase, wherein said transgenic offspring expresses the delta-12 desaturase and the at least one or more enzymes of unsaturated fatty acid metabolism,
wherein the plant metabolic property is production of polyunsaturated fatty acids comprising
VLC-PUFAs,
wherein the transgenic plant exhibits a negative correlation between seed oil content and delta-12-desaturase conversion efficiency whether grown in greenhouse or in field conditions, and wherein VLC-PUFA production levels in the transgenic plant and said selected transgenic offspring is consistent across different environmental conditions as compared to a corresponding control plant not expressing said delta-12-desaturase and grown under similar environmental conditions.
2. The assay method according to claim 1 , wherein the Delta-12 desaturase is obtained from an organism of class oomycetes.
3. The assay method of claim 1 , wherein the delta-12 desaturase expressed by the transgenic plant has 100% total amino acid sequence identity to the amino acid sequence as set forth in SEQ ID NO: 328.