Granted Patent
B2
US 12,723,252 · App. 18/479,876 · Granted Sep 1, 2026
Materials and methods for increasing the tocopherol content in seed oil
Assignee:
BASF PLANT SCIENCE COMPANY GMBH
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Abstract
The present invention relates generally to the field of molecular biology and concerns increasing the tocopherol content of a plant relative to a control plant, comprising expressing in a plant at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase. The present invention also relates to methods for the manufacture of oil, fatty acid- or lipids-containing compositions, and to such oils and lipids as such.
Claims (11)
1 . A method for producing a Brassica napus oil comprising n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) and tocopherol, comprising:
(i) providing transgenic Brassica napus seeds comprising a biosynthetic pathway that synthesizes eicosapentaenoic acid (EPA), docosapentaenoic acid (DPA), and docosahexaenoic acid (DHA), wherein each transgenic seed comprises multiple expression cassettes comprising transgenes which comprise at least one polynucleotide encoding a delta-12-desaturase, at least one polynucleotide encoding a delta-6-desaturase, at least one polynucleotide encoding a delta-6-elongase, and at least one polynucleotide encoding a delta-5-desaturase; and
(ii) obtaining seed oil from the transgenic seeds, wherein said seed oil comprises increased tocopherol content and increased n-3 very long chain polyunsaturated fatty acid (VLC-PUFA) content as compared to control Brassica napus seeds, wherein the increased n-3 VLC-PUFA content comprises EPA, DPA, and/or DHA,
wherein the increased tocopherol content and the increased n3 VLC-PUFA content in the transgenic seeds are positively correlated, and
wherein the increased tocopherol content comprises increased total tocopherol content, and the increased total tocopherol content is the result of the biosynthetic pathway that synthesizes EPA, DPA, and DHA.
2 . The method of claim 1 , wherein the total transgenic seed content of tocopherol is more than 35 mg/100 g seed.
3 . The method of claim 1 , wherein the total transgenic seed content of tocopherol is from about 36 mg/100 g seed to about 44 mg/100 g seed.
4 . The method of claim 1 , wherein the amount of VLC-PUFAs in the transgenic seed is increased by at least 5%, at least 10%, at least 15%, at least 20%, or at least 30% by weight compared to seed from a wild-type Brassica napus control plant.
5 . The method of claim 1 , wherein the amount of VLC-PUFAs in the transgenic seed is increased by 5% to 30% by weight compared to seed from a wild-type Brassica napus control plant.
6 . The method of claim 1 , wherein the tocopherol content is measured as the numeric average of transgenic seed tocopherol content of at least 100 Brassica napus plants.
7 . The method of claim 1 , wherein the increased n-3 VLC-PUFA content comprises DHA and wherein the increased tocopherol content and the increased DHA content in the transgenic seed are positively correlated.
Continuity (6)
Provisional Application
62234373
· Sep 29, 2015
Provisional Application
62079622
· Nov 14, 2014
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