Optical measurement device and optical measurement method
In multiplex CARS, a fingerprint region is a region where signals are densely concentrated. Information about the intensities and spatial distribution of these signals is important in cell analysis. However, there has been a problem in that the signal intensities are low. Accordingly, mechanisms 702 and 703 for adjusting the power branching ratio between light that enters a photonic crystal fiber 705 and pumping light; mechanisms 710 and 711 for adjusting the divergence/convergence state of the pumping light; and mechanisms 706 and 2101 for adjusting the divergence/convergence state of Stokes light are provided to enable adjustment for emphasizing a desired wavelength band.
1. An optical measurement device comprising:
a short pulse laser light source;
a splitter configured to split emitting light from the short pulse laser light source into a first light flux and a second light flux;
an optical fiber configured to generate supercontinuum light from the first light flux;
a dichroic mirror which combines a long wavelength component of the supercontinuum light serving as Stokes light with the second light flux serving as pumping light;
a focus optical system including an objective lens configured to focus light combined in the dichroic mirror onto a sample;
a spectroscope configured to detect light generated from the sample; and
a movable stage including a motor, a controller, and a collimator lens, the controller-being configured to move the movable stage using the motor to adjust a focal point of the Stokes light in the sample using the collimator lens based on an intensity of a desired band of a spectrum detected in the spectroscope, and to adjust the focal point of the Stokes light using the collimator lens with respect to but without affecting the pumping light so as to change a combined state between the Stokes light and the pumping light.
2. The optical measurement device according to claim 1 , comprising:
a second optical fiber configured to cause the second light flux serving as the pumping light to pass.
3. The optical measurement device according to claim 1 , wherein the adjustment of the focus position of the Stokes light in the sample is performed by adjusting in a direction of an optical axis a position of a lens provided in an optical path for the Stokes light.
4. The optical measurement device according to claim 1 ,
wherein the adjustment of the focus position of the Stokes light in the sample is performed at a plurality of wavenumber bands, and
wherein measurement results at the plurality of wavenumber bands are connected and displayed.