Milk-based protein hydrolysates and compositions made thereof
View Patent ↗A process for preparing a milk protein hydrolysate comprising hydrolysing a milk-based proteinaceous material with a microbial alkaline serine protease in combination with bromelain, a protease from Aspergillus and a protease from Bacillus.
1. A process for preparing a milk protein hydrolysate, the process comprising hydrolyzing a milk-based proteinaceous material with subtilisin Carlsberg, bromelain, leucine aminopeptidase from Apergillus oryzae , aspergillopepsin 1 from Apergillus oryzae , and neutral proteinase from Bacillus subtilis.
2. The process according to claim 1 comprising:
a first hydrolysis step comprising hydrolysing the milk-based proteinaceous material with subtilisin Carlsberg; and
a second hydrolysis step comprising hydrolysing the milk protein with the bromelain, the leucine aminopeptidase, the Aspergillopepsin 1 and the neutral proteinase from Bacillus subtilis.
3. The process according to claim 1 , wherein the milk-based proteinaceous material is selected from the group consisting of whey protein, casein and mixtures thereof.
4. The process according to claim 1 , wherein the milk-based proteinaceous material is whey protein.
5. The process according to claim 1 further comprising subjecting the hydrolyzed milk-based proteinaceous material to a process selected from the group consisting of enzyme inactivation, microfiltration and ultrafiltration.
6. The process according to claim 1 , wherein the milk protein hydrolysate is an extensively hydrolyzed product, and the extent of hydrolysis (NPN/TN %) is greater than 95%.
7. The process according to claim 1 , wherein the extent of hydrolysis (NPN/TN %) is greater than 99%.