Composition suitable for protecting microorganisms
View Patent ↗The present invention relates to a composition comprising at least one carrier comprising a polysaccharide, at least one antioxidant and at least one amino acid selected from cysteine, lysine, alanine and arginine. It also relates to the use of such a composition for the protection of microorganisms during drying, storage and/or reconstitution, to a culture powder, to a process of making the culture powder and to products comprising the culture powder.
1. A process for preparing a culture powder, the process comprising:
a. producing a biomass by fermentation with live bacteria;
b. concentrating the biomass obtained in step a);
c. conditioning the concentrated biomass with an aqueous solution of a composition comprising a carrier material comprising a polysaccharide, the composition further comprising at least one antioxidant and an amino acid selected from the group consisting of (i) cysteine and alanine; (ii) cysteine, lysine, and alanine; (iii) lysine and arginine; (iv) cysteine and arginine; (v) cysteine, lysine, and arginine; (vi) lysine, alanine, and arginine; and (vii) cysteine, arginine, and alanine;
wherein any lysine is 8 to 20% of the composition based on dry weight, any alanine is 8 to 20% of the composition based on dry weight, any arginine is 8 to 20% of the composition based on dry weight, and any cysteine is 2 to 10% of the composition based on dry weight; and
d. spray-drying the conditioned biomass to form the culture powder, the culture powder comprising the live bacteria and a matrix comprising the composition in a matrix: live bacteria dry weight ratio of at least one.
2. The process according to claim 1 , wherein step c) comprises the following sub-steps:
adding the aqueous solution to the biomass to form a biomass solution such as to achieve a concentration of the composition of 40-60 wt %, based on the total dry weight of the composition and the biomass;
maintaining the biomass and the composition contact in the biomass solution for a duration of 20 to 150 minutes; and
adjusting the pH of the biomass solution between 6.5 and 8.5.
3. The process according to claim 1 , wherein step c) is carried out in a continuous process.
4. The process according to claim 2 , wherein the pH of the biomass solution is between 6.8 and 7.2.