Polypeptides having endoglucanase activity and polynucleotides encoding same
The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
1. A nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more heterologous control sequences that direct the production of the polypeptide, and wherein the polypeptide having endoglucanase activity is a polypeptide having at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2; or a polypeptide encoded by a polynucleotide having at least 98% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
2. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
3. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises (i) the polypeptide of SEQ ID NO: 2; (ii) amino acids 18 to 414 of the polypeptide of SEQ ID NO: 2.
4. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity comprises amino acids 18 to 414 of the polypeptide of SEQ ID NO: 2.
5. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide having at least 99% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
6. The nucleic acid construct of claim 1 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide comprising nucleotides 52 to 1242 of SEQ ID NO: 1.
7. An isolated recombinant host cell transformed with the nucleic acid construct of claim 1 .
8. A method of producing a polypeptide having endoglucanase activity, comprising cultivating the recombinant host cell of claim 7 under conditions conducive for production of the polypeptide and optionally recovering the GH61 polypeptide.
9. An isolated recombinant host cell transformed with a nucleic acid construct comprising a polynucleotide encoding a polypeptide having endoglucanase activity, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide, wherein the polynucleotide encoding the polypeptide is heterologous to the recombinant host cell, and wherein the polypeptide having endoglucanase activity is a polypeptide having at least 98% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2; or a polypeptide encoded by a polynucleotide having at least 98% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
10. The recombinant host cell of claim 9 , wherein the polypeptide having endoglucanase activity has at least 99% sequence identity to amino acids 18 to 414 of SEQ ID NO: 2.
11. The recombinant host cell of claim 9 , wherein the polypeptide having endoglucanase activity comprises (i) the polypeptide of SEQ ID NO: 2; (ii) amino acids 18 to 414 of the polypeptide of SEQ ID NO: 2.
12. The recombinant host cell of claim 9 , wherein the polypeptide having endoglucanase activity comprises amino acids 18 to 414 of the polypeptide of SEQ ID NO: 2.
13. The recombinant host cell of claim 9 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide having at least 99% sequence identity to nucleotides 52 to 1242 of SEQ ID NO: 1.
14. The recombinant host cell of claim 9 , wherein the polypeptide having endoglucanase activity is encoded by a polynucleotide comprising nucleotides 52 to 1242 of SEQ ID NO: 1.
15. A method of producing a polypeptide having endoglucanase activity, comprising cultivating the recombinant host cell of claim 9 under conditions conducive for production of the polypeptide, and optionally recovering the polypeptide.