IP Library › Granted Patent US 10,487,123
Granted Patent B2
US 10,487,123 · App. 15/656,616 · Granted Nov 26, 2019

Chimeric insecticidal proteins toxic or inhibitory to lepidopteran pests

Inventors: James A. Baum (Webster Groves, MO); Thomas A. Cerruti (Newton, MA); Crystal L. Dart (Norton, MA); Leigh H. English (Chesterfield, MO); Stanislaw Flasinski (Chesterfield, MO); Xiaoran Fu (Belmont, MA); Victor M. Guzov (Cambridge, MA); Arlene R. Howe (Chesterfield, MO); Jay P. Morgenstern (Boston, MA); James K. Roberts (Chesterfield, MO); Sara A. Salvador (Wildwood, MO); Jinling Wang (Belmont, MA)
Assignee: Monsanto Technology LLC
C07K14/325A01N37/46A01N63/02C12N15/8286C07K2319/00C12N15/62Y02A40/162
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Quick Facts
Patent No.
US 10,487,123
App. No.
15/656,616
Granted
Nov 26, 2019
Kind
B2
Abstract

Nucleotide sequences are disclosed that encode novel chimeric insecticidal proteins exhibiting Lepidopteran inhibitory activity. Particular embodiments provide compositions and transformed plants, plant parts, and seeds containing the recombinant nucleic acid molecules encoding one or more of the chimeric insecticidal proteins.

Claims (26)

1. A chimeric insecticidal protein comprising the amino acid sequence as set forth in SEQ ID NO: 99 wherein the chimeric insecticidal protein exhibits inhibitory activity against an insect species of the order Lepidoptera.

2. A polynucleotide encoding the chimeric insecticidal protein of claim 1 , wherein the polynucleotide is operably linked to a heterologous promoter.

3. A polynucleotide encoding a chimeric insecticidal protein, wherein the polynucleotide comprises a nucleotide sequence that a) is set forth in any of SEQ ID NO: 98 or 124, or b) encodes the chimeric insecticidal protein of claim 1 .

4. A host cell comprising the polynucleotide of claim 3 , wherein the host cell is selected from the group consisting of a bacterial host cell and a plant host cell.

5. The host cell of claim 4 , wherein the bacterial host cell is selected from the group consisting of Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella , and Erwinia.

6. The host cell of claim 4 , wherein the plant host cell is selected from the group of plants consisting of monocots and dicots.

7. An insect inhibitory composition comprising the chimeric insecticidal protein of claim 1 .

8. The insect inhibitory composition of claim 7 , further comprising at least one insect inhibitory agent different from the chimeric insecticidal protein.

9. The insect inhibitory composition of claim 8 , wherein the at least one insect inhibitory agent is selected from the group consisting of an insect inhibitory protein and an insect inhibitory dsRNA molecule.

10. The insect inhibitory composition of claim 8 , wherein the at least one other pesticidal agent exhibits activity against one or more pest species of the orders Lepidoptera, Coleoptera, Hemiptera, Homoptera, or Thysanoptera.

11. A seed comprising an insect inhibitory effective amount of:

a) the chimeric insecticidal protein of claim 1 ; or

b) the polynucleotide set forth in SEQ ID NO: 98 or 124.

12. A method of controlling a Lepidopteran pest, the method comprising contacting the Lepidopteran pest with an inhibitory amount of the chimeric insecticidal protein of claim 1 .

13. A transgenic plant cell, plant or plant part comprising a chimeric insecticidal protein, wherein the chimeric insecticidal protein comprises SEQ ID NO: 99.

14. A method of controlling a Lepidopteran pest, comprising exposing the pest to the transgenic plant cell, plant or plant part of claim 13 , wherein said plant cell, plant or plant part expresses a Lepidopteran inhibitory amount of the chimeric insecticidal protein.

15. A commodity product derived from the plant cell, plant, or plant part of claim 13 , wherein the product comprises a detectable amount of the chimeric insecticidal protein.

16. The commodity product of claim 15 , wherein the product is selected from the group consisting of plant biomass, oil, meal, animal feed, flour, flakes, bran, lint, hulls, and processed seed.

17. A method of producing a seed comprising the chimeric insecticidal protein of claim 1 , the method comprising:

(a) planting at least one seed comprising the chimeric insecticidal protein of claim 1 ;

(b) growing plants from the seed; and

(c) harvesting seed from said plants, wherein the harvested seed comprises the chimeric insecticidal protein of claim 1 .

18. A recombinant polynucleotide molecule encoding the chimeric insecticidal protein of claim 1 , said molecule comprising the full length nucleotide sequence of SEQ ID NO: 98 or 124; and a polynucleotide sequence encoding an insect inhibitory agent different from said chimeric insecticidal protein.

19. A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a chimeric insecticidal protein, wherein:

a. the chimeric insecticidal protein comprises SEQ ID NO: 99; or

b. the polynucleotide segment of SEQ ID NO: 98 or 124.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jul 21, 2017
From: BAUM, JAMES A.; CERRUTI, THOMAS A.; DART, CRYSTAL L.; ENGLISH, LEIGH H.; FU, XIAORAN; GUZOV, VICTOR M.; HOWE, ARLENE R.; MORGENSTERN, JAY P.; SALVADOR, SARA A.; WANG, JINLING; FLASINSKI, STANISLAW; ROBERTS, JAMES K.
To: MONSANTO TECHNOLOGY LLC
Reel/Frame 043067/0299 →
Continuity (3)
Continuation In Part 14884469 · Oct 15, 2015
Provisional Application 62064989 · Oct 16, 2014
Related Publication 20170327547A1 · Nov 16, 2017
Cited By (2)
US 12,264,182 US 12,668,613