Granted Patent B2

US 12,668,613 · App. 18/633,982 · Granted Jun 30, 2026

Inventors: James A. Baum (Webster Groves, MO); Thomas A. Cerruti (Newton, MA); Crystal L. Dart (Norton, MA); Leigh H. English (Chesterfield, MO); Stanislaw Flasinski (Ballwin, MO); Xiaoran Fu (Belmont, MA); Victor M. Guzov (Cambridge, MA); Arlene R. Howe (Clarkson Valley, MO); Jay P. Morgenstern (Cambridge, MA); James K. Roberts (Chesterfield, MO); Sara A. Salvador (Wildwood, MO); Jinling Wang (Belmont, MA)

Assignee: Monsanto Technology LLC

C07K14/325C12N15/8286C07K2319/00C12N15/62Y02A40/146

Abstract

Claims (22)

1 . A chimeric insecticidal protein comprising the amino acid sequence as set forth in SEQ ID NO: 109 or comprising an amino acid sequence having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:109.

2 . The chimeric insecticidal protein of claim 1 , wherein the chimeric insecticidal protein comprises the amino acid sequence as set forth in SEQ ID NO:109.

3 . A polynucleotide encoding the chimeric insecticidal protein according to claim 1 , wherein the polynucleotide is operably linked to a heterologous promoter.

4 . A host cell comprising the polynucleotide set forth in claim 3 , wherein the host cell is selected from the group consisting of a bacterial host cell and a plant host cell.

5 . The host cell of claim 4 , wherein the bacterial host cell is selected from the group consisting of Agrobacterium, Rhizobium, Bacillus, Brevibacillus, Escherichia, Pseudomonas, Klebsiella , and Erwinia ; and wherein the Bacillus species is a Bacillus cereus or a Bacillus thuringiensis , said Brevibacillus is a Brevibacillus laterosporus , and said Escherichia is an Escherichia coli.

6 . The host cell of claim 4 , wherein the plant host cell is selected from the group of plants consisting of monocots and dicots.

7 . An insect inhibitory composition comprising the chimeric insecticidal protein according to claim 1 .

8 . The insect inhibitory composition of claim 7 , further comprising at least one insect inhibitory agent different from the chimeric insecticidal protein.

9 . The insect inhibitory composition of claim 8 , wherein the at least one insect inhibitory agent is selected from the group consisting of an insect inhibitory protein and an insect inhibitory dsRNA molecule.

10 . The insect inhibitory composition of claim 8 , wherein the at least one insect inhibitory agent exhibits activity against one or more pest species of the orders Lepidoptera , Coleoptera, Hemiptera, Homoptera, or Thysanoptera.

11 . A seed comprising an insect inhibitory effective amount of the chimeric insecticidal protein of claim 1 .

12 . A method of controlling a Lepidopteran pest, the method comprising contacting the Lepidopteran pest with an inhibitory amount of the chimeric insecticidal protein of claim 1 .

13 . A recombinant nucleic acid molecule comprising a heterologous promoter operably linked to a polynucleotide segment encoding a chimeric insecticidal protein, wherein the chimeric insecticidal protein comprises an amino acid sequence having at least 95% identity to the amino acid sequence of SEQ ID NO: 109.

14 . A transgenic plant cell, plant or plant part comprising the recombinant nucleic acid molecule of claim 13 .

15 . A method of controlling a Lepidopteran pest, comprising exposing the pest to the transgenic plant cell, plant or plant part of claim 14 , wherein said plant cell, plant or plant part expresses a Lepidopteran inhibitory amount of the chimeric insecticidal protein.

16 . A commodity product derived from the plant cell, plant, or plant part of claim 14 , wherein the product comprises a detectable amount of the chimeric insecticidal protein.

17 . The commodity product of claim 16 , wherein the product is selected from the group consisting of plant biomass, oil, meal, animal feed, flour, flakes, bran, lint, hulls, and processed seed.

18 . A method of producing a seed comprising the chimeric insecticidal protein of claim 1 , the method comprising:

a) planting at least one seed comprising the chimeric insecticidal protein of claim 1 ;

b) growing plants from the seed; and

c) harvesting seed from the plants, wherein the harvested seed comprises the chimeric insecticidal protein of claim 1 .

19 . A recombinant polynucleotide molecule encoding the chimeric insecticidal protein of claim 1 , wherein said recombinant polynucleotide molecule comprises the nucleotide sequence of SEQ ID NO:129.

Continuity (6)

References Cited (183)