Sharply resolving labeled protein molecular weight standards
Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced.
1. A pre-labeled protein standard set comprising a plurality of labeled proteins, wherein one or more of the plurality of labeled proteins is a selectively labeled protein comprising a labeling compound on a first or a target amino acid residue and lacks amino acid residues of a second or non-target amino acid residue that can react with said labeling compound,
wherein at least one or more of said plurality of labeled proteins has an amino acid sequence that is at least 95% identical to the amino acid sequence selected from the group consisting of SEQ ID NOs: 11, 12, 14, 15, 16, 17, 38, 39, and 41.
2. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is lysine and said second or non-target amino acid residue is one or more of cysteine, histidine, or tryptophan.
3. The pre-labeled protein standard set of claim 2 , wherein said first or target amino acid residue is lysine and said second or non-target amino acid residue is cysteine.
4. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is cysteine and said second or non-target amino acid residue is one or more of lysine, histidine, or tryptophan.
5. The pre-labeled protein standard set of claim 4 , wherein said first or target amino acid residue is cysteine and said second or non-target amino acid residue is lysine.
6. The pre-labeled protein standard set of claim 1 , wherein wherein said first or target amino acid residue is glutamate and said second or non-target amino acid residue is aspartate.
7. The pre-labeled protein standard set of claim 1 , wherein wherein said first or target amino acid residue is aspartate and said second or non-target amino acid residue is glutamate.
8. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is histidine and said second or non-target amino acid residue is one or more of cysteine, lysine, or tryptophan.
9. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is tryptophan and said second or non-target amino acid residue is one or more of cysteine, lysine, histidine or asparagine.
10. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is asparagine and said second or non-target amino acid residue is one or more of cysteine, lysine, histidine or tryptophan.
11. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is tyrosine and said second or non-target amino acid residue is one or more of cysteine, lysine, histidine or tryptophan.
12. The pre-labeled protein standard set of claim 1 , wherein said first or target amino acid residue is methionine and said second or non-target amino acid residue is one or more of cysteine, lysine, histidine or tryptophan.
13. The pre-labeled protein standard set of claim 1 , wherein said labeling compound is a chromophore, a fuorophore or a dye.
14. The pre-labeled protein standard set of claim 13 , wherein said dye is a textile dye.
15. The pre-labeled protein standard set of claim 14 , wherein said textile dye is remazol brilliant blue R, uniblue A, malachite green isothiocyanite, or orange 16 .
16. The pre-labeled protein standard set of claim 13 , wherein said fluorophore is an Alexa fluor dye, a BODIPY dye, fluorescein or a derivative thereof, eosin or a derivative thereof, tetramethylrhodamine, rhodamine or a derivative thereof, Texas red or a derivative thereof, pyridyloxazole or a derivative thereof, NBD chloride, NBD fluoride, ABD-F, lucifer yellow or a derivative thereof, 8-anilino-1-napthalenesulfonic acid (8-ANS) or a derivative thereof, or Oregon green or a derivative thereof.
17. The pre-labeled protein standard set of claim 1 , wherein said plurality of labeled proteins are provided in one or more solutions and wherein said one or more solutions comprise at least one buffer, at least one detergent, at least one reducing agent, at least one alcohol, or at least one dye.
18. A kit comprising:
the pre-labeled protein standard set of claim 1 ; and
one or more of: one or more gel cassettes, a gel loading buffer, a gel loading dye, a reducing agent, a blotting membrane, a lysis buffer, a purification matrix, or an unlabeled protein standard set.