Compositions containing combinations of bioactive molecules derived from microbiota for treatment of disease
Compositions consisting of bioactive molecules derived from the microbiota of a mammal are provided herein. When administered orally with a colonic delivery system, the compositions are useful for the prophylaxis and treatment of diseases, in particular inflammatory, autoimmune and infectious diseases. The compositions comprise combinations of small molecules and bacterial antigens formulated in colonic delivery systems. Use of the compositions results in any or all of: induction of immune tolerance; strengthening of the gut mucosal barrier integrity; reduction of inflammation; and amelioration of a disease state caused by inflammation, an autoimmune reaction or an infectious agent.
1. A composition that induces proliferation and/or accumulation of regulatory T cells, the composition comprising: (a) one or more short-chain fatty acid or short-chain fatty acid derivative; and (b) one or more protein antigens purified from bacteria belonging to Clostridium Cluster IV, Clostridium Cluster XIVa, or Clostridium Cluster XVIII.
2. The composition of claim 1 , wherein (a) is selected from the group consisting of butyrate, isobutyrate, proprionate, acetate, tributyrin, pivaloyloxymethyl butyrate, and monoacetone glucose 3-butyrate.
3. The composition of claim 1 , wherein (a) is butyrate.
4. The composition of claim 1 , wherein (a) is a mixture of salts of butyrate, isobutyrate, propionate, and acetate.
5. The composition of claim 1 , further comprising a pH sensitive composition comprising an enteric polymer.
6. A pharmaceutical composition comprising the composition according to claim 1 and a pharmaceutically acceptable component.
7. A method for inducing proliferation and/or accumulation of regulatory T cells in an individual in need thereof, the method comprising administering, to the individual, the composition of claim 1 .
8. A method of treating, aiding in treating, reducing the severity of, or preventing at least one disease selected from an autoimmune disease, an inflammatory disease, an allergic disease, and an infectious disease in an individual in need thereof, the method comprising administering the composition of claim 1 to the individual.